Autoradiography (Fluorography)
After the plate has been developed, spray twice with National Diagnostics' Autofluor and allow the plate to dry. This impregnates the plate with phosphors, which will convert the b emissions to more readily detectable photons. Autofluor enhances the speed and sensitivity of detection when the plate is placed on film. Follow this
link for details on autoradiography procedures.
Liquid scintillation counting of scraped TLC silica
Option 1: Suspend the silica powder in 10ml of a cocktail which has a gel phase with water such as National Diagnostics'
Hydrofluor. Shake well, and add 3ml H
2O. Shake until gel forms, and count.
Option 2: Suspend the silica in 10ml of an organic based (non-emulsion) cocktail such as National Diagnostics'
Ecoscint O. Add 0.5-1g finely divided silica thixotrophic agent to form a clear gel in the solution, keeping the TLC particles suspended. Count as usual.
