Gel Prep for Native PAGE of DNA

Native PAGE gels are prepared by mixing an Acrylamide/Bisacrylamide monomer concentrate (AccuGel 19:1 or 29:1), buffer concentrate and water to achieve the desired gel concentration. TEMED and Ammonium Persulfate are added to initiate polymerization and the solution is poured into a cassette. The comb is then inserted.

The cassette is formed by two glass plates separated by spacers, typically 0.5-1mm in thickness, and sealed at the bottom (See figure below or follow this link for a more detailed discussion of the electrophoresis apparatus). The polymerization process takes 1-2 hours to complete.

An exploded view of a gel cassette.

The table below gives solution amounts to prepare gels of various percentages and buffers.

Prepare solution of monomer, buffer and water as per table.

De-gas if desired.

Add APS and TEMED.

Pour gel.

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Products Related to this Discussion:

AccuGel 29:1
Concentrated solution of acrylamide and bis-acrylamide (29:1) for native DNA electrophoresis and SDS-PAGE. Filtered, deionized, and stabilized.

Ammonium Persulfate - ULTRA PURE
Exceeds ACS standards. Low absorbed water results in consistent initiation.

TBE Buffer (10X)
Formulated with 18MegOhm water. 0.2 micron filtration. The most stable TBE on the market.

TEMED - ULTRA PURE
Fractionally distilled to remove all trace metals and amine impurities. Stored under nitrogen.

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