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ProtoBlue Safe

  • The Best Performing Colloidal Stain - Detects as little as 5ng protein
Compared to similar stains, the more finely controlled colloidal structure of ProtoBlue Safe improves both the sensitivity and the universality of staining. ProtoBlue Safe is less prone to high background caused by trace residual SDS in the gel.
  • Costs Less than Regular Coomassie
Laboratories typically spend twice as much per gel in the methanol and acetic acid for staining and destaining with regular Coomassie than for ProtoBlue Safe (including ethanol cost). In addition, ProtoBlue Safe is much faster, more sensitive, and you can pour the used stain solution down the drain. Protein visualization is faster, safer, more sensitive and less-expensive with ProtoBlue Safe.
  • Nonhazardous Disposal
Used stain solution is not a hazardous waste (as defined by United States Title 40 Code of Federal Regulations (40 CFR 261.24(a)).
  • Fast Protocol - No Methanol or Acetic Acid

Catalog Number: EC-722
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APPLICATIONS OF PROTOBLUE SAFE
Denaturing Protein Electrophoresis: SDS-PAGE
Comparing Protein Stains
Staining Protein Gels with Colloidal Coomassie
Related Products   
ProtoGel Quick-Cast 12%
Ready-to-use gel solution for SDS-PAGE that combines the convenience of pre-cast gels with the economy of casting your own.
 
ProtoGel (30%)
30% concentrated solution of acrylamide and bis-acrylamide, 37.5 : 1 ratio. Filtered, deionized, and stabilized.
 
ProtoGel (40%)
Concentrated solution of acrylamide and bis-acrylamide, 37.5 : 1 ratio. Filtered, deionized, and stabilized.
 
AccuGel 29:1
Concentrated solution of acrylamide and bis-acrylamide (29:1) for native DNA electrophoresis and SDS-PAGE. Filtered, deionized, and stabilized.
 
Tris-Glycine-SDS PAGE Buffer (10X)
Ultra-pure concentrated standard running buffer for SDS-PAGE. Formulated with 18 MegOhm water. 0.2 micron filtration.
 
ProtoGel Resolving Buffer (4X)
4X Laemmli Tris-HCl resolving gel buffer for clear, reproducible SDS-PAGE.
 
ProtoGel Stacking Buffer
4X Laemmli Tris-HCl stacking gel buffer for clear, reproducible SDS-PAGE.
 
Protein Loading Buffer Blue (2X)
Ready to use sample buffer for SDS-PAGE.
 
Protein Loading Buffer Blue (2X)
Ready to use sample buffer for SDS-PAGE.
 
Tris-Tricine-SDS PAGE Buffer (10X)
Alternative tank buffer for the SDS-PAGE separation of small proteins.
 
ProtoMarkers
Broad range pre-stained protein markers for SDS-PAGE.
 
Ammonium Persulfate - ULTRA PURE
Exceeds ACS standards. Low absorbed water results in consistent initiation.
 
TEMED - ULTRA PURE
Fractionally distilled to remove all trace metals and amine impurities. Stored under nitrogen.
 

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