Electrophoresis
AquaPor ES GTAC Agarose
$68.00 – $244.00
Quantity discount when you buy 4 or more 100g units!
- Ultra-High Strength/Low EEO Agarose
- Blotting of MegaBase DNA
- Finest Particle Size for Fastest Dissolution
Description
Quantity discount when you buy 4 or more 100g units!
- Ultra-High Strength/Low EEO Agarose
- Blotting of MegaBase DNA
- Finest Particle Size for Fastest Dissolution
The very low EEO of AquaPor ES will significantly reduce electrophoresis times for pulsed field gel (PFG) applications, and also increase band sharpness. The extremely high gel strength makes it possible to cast gels as low as 0.3% which remain intact through staining, destaining, photographic documentation and blotting. These low percentage gels may be used for the electrophoretic separation of DNA fragments as large as 50 kb, and macromolecular protein complexes from 500 kDa to 10 MDa size. Ultra-fine particle size ensures the fastest dissolution for easiest gel preparation.
| EEO (-mr) | Gel Strength (g/cm2) | Gel Temp (°C) |
| ≤ 0.05 | ≥ 3200 @ 1.5% | ≤ 40 @ 1.0% |
| Melt Temp (°C) | Sulfate (%) | DNase, RNase, Protease |
| ≤ 89 @ 1.5% | ≤ 0.20 | None |
Additional information
| Weight | 0.2 lbs |
|---|---|
| Dimensions | 6 × 3 × 3 in |
Protocol
DISSOLVING AQUAPOR AGAROSES
- Add room temperature buffer to a flask that is 2.5 – 4 times the volume of gel solution. Add a teflon-coated stir bar.
- Add AquaPor powder while stirring vigorously so the agarose is dispersed uniformly. Stir for 2 minutes to hydrate the agarose.
- Tare the flask and solution.
- Place in a microwave oven and heat at 100% power using 20 – 60 second intervals. Swirl gently between intervals to resuspend the agarose.
- Continue the cycle of heating and swirling until the agarose is completely dissolved (no visible particles are present).
- Add distilled water to obtain the initial weight and mix.
- Cool the solution to 50 – 60°C before pouring the gel.
* Chill the gel for 30 minutes prior to comb removal when using AquaPor LM, HR, and low (<1%) concentration of AquaPor LE and ES.
Safety Overview
Appearance and Odor
White powder
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
AS PART OF GOOD INDUSTRIAL AND PERSONAL HYGIENE AND SAFETY PROCEDURE, AVOID ALL UNNECESSARY EXPOSURE TO THE CHEMICAL SUBSTANCE AND ENSURE PROMPT REMOVAL FROM SKIN, EYES, AND CLOTHING.
- UV Shadowing
- Using PAGE to Determine Nucleic Acid Molecular Weight
- Uneven Staining
- The Polyacrylamide Matrix-Buffer Strength
- The Polyacrylamide Matrix
- The Mechanical and Electrical Dynamics of Gel Electrophoresis — Electrophoresis System Dynamics
- The Mechanical and Electrical Dynamics of Gel Electrophoresis – Ohm’s Law
- The Mechanical and Electrical Dynamics of Gel Electrophoresis – Intro and Sample Mobility
- The Electrophoresis Matrix
- The Agarose Matrix
- Staining Proteins Immobilized on Membranes
- Staining Protein Gels with Coomassie Blue
- SSCP Analysis
- Southern Blotting
- Smeared Bands
- Silver Staining Protein Gels
- Silver Staining DNA Gels
- Sanger Sequencing
- Sample Preparation for SDS-PAGE
- Sample Preparation for Native Protein Electrophoresis
- Sample Preparation for Native PAGE of DNA
- Sample Prep for Denaturing PAGE of DNA
- S1 Mapping
- Run Conditions in Denaturing PAGE
- RNA Mapping
- RNA Electrophoresis
- Ribonuclease Protection
- Restriction Digest Mapping
- Radioactive Emissions and the Use of Isotopes in Research
- Protein Fixation on Gels
- Primer Extension
- Preparing Denaturing DNA & RNA Gels
- Preparation of Denaturing Agarose Gels
- Preparation of Agarose Gels
- Pouring Sequencing Gels
- Post-Electrophoretic Visualization with Nuclistain
- PFGE and FIGE
- Peptide Mapping
- PCR Analysis: Yield and Kinetics
- PCR Analysis: An Examination
- Overview of Western Blotting
- Northern Blotting
- Native Protein Electrophoresis
- Native PAGE of DNA
- Multiphasic Buffer Systems
- Mobility Shift Assay
- Methylation & Uracil Interference Assays
- Method for Western Blotting
- Mechanism of Immunostaining
- Mechanism of Immunostaining
- Measuring Molecular Weight with SDS-PAGE
- Maxam & Gilbert Sequencing
- Manual Sequencing
- Isotachophoresis
- Isoelectric Focusing
- In Gel Enzyme Reactions
- Immunostaining with Alkaline Phosphatase
- Immuno-Electrophoresis / Immuno-Diffusion
- Horizontal and Vertical Gel Systems – Vertical Tube Gels
- Horizontal and Vertical Gel Systems – The Vertical Slab Gel System
- Horizontal and Vertical Gel Systems – The Horizontal Gel System
- Homogeneous Buffer Systems
- Heteroduplex Analysis
- Guide Strip Technique
- Gel Preparation for Native Protein Electrophoresis
- Gel Preparation for Native PAGE of DNA
- Gel Electrophoresis of RNA & Post Electrophoretic Analysis
- Gel Electrophoresis of PCR Products
- Faint bands, low background
- Faint Bands, High Background
- Ethidium Bromide Staining
- Enzyme Linked Immunosorbent Assay (ELISA)
- Electrophoresis Buffers-Choosing the Right Buffer
- Electrophoresis Buffers–The Henderson-Hasselbalch Equation
- DNase I Footprinting
- DNA/RNA Purification from PAGE Gels
- DNA/RNA Purification from Agarose Gels – Electroelution
- Differential Display
- Denaturing Protein Electrophoresis: SDS-PAGE
- Denaturing Polyacrylamide Gel Electrophoresis of DNA & RNA
- Coomassie Blue Stain- Troubleshooting
- Conformational Analysis
- Casting Gradient Gels
- Buffer Additives-Surfactants
- Buffer Additives-Reducing Agents
- Buffer Additives-Hydrogen Bonding Agents
- Blotches on Gel
- Biological Macromolecules: Nucleic Acids
- Biological Macromolecules – Proteins
- Autoradiography
- Autoradiographic Enhancement with Autofluor
- Automated Sequencers
- Analysis of DNA/Protein Interactions
- An Overview of Northern and Southern Blotting
- Alkaline Blotting
- Agarose Gel Electrophoresis of DNA and RNA – Uses and Variations
- Agarose Gel Electrophoresis of DNA and RNA – An Introduction
- Activity Stains
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