Gel Electrophoresis of Proteins

Agarose electrophoresis of RNA requires the inclusion of denaturing agents in the gel. In the absence of denaturants, RNA assumes compact secondary structures, which distort the relationship between molecular weight and mobility.Urea, used as a denaturant in polyacrylamide gels, disrupts the hydrogen bonds which hold the agarose gel together, and alkaline conditions, used in denaturing…

Isotachophoresis is a method of electrophoresis that employs the basic principles of the stacking gel phase of multiphasic systems discussed in the preceding section. Employing nonsieving media, often low percentage polyacrylamide, isotachophoresis in its simplest form can be thought of as a stacking gel alone, without the separation gel. The principles are the same. With…