Protein Detection

Blotches can be caused by overhandling of the gel, or by handling the gel without gloves. This can create uneven surfaces or protein deposits that can bind Coomassie to the gel. To minimize this problem, handle the gel as little as possible, and always with clean gloves.   This gel can be recovered in most…

Uneven staining is almost always the result of insufficient agitation during staining.  The gel is initially more dense than the staining solution and will tend to sink to the bottom of the dish.  Some portions of the gel will have stains under them, others will not, and the gel will show darker and lighter areas…