Blotting and Detection Buffers
Quantity discount when you buy 4 or more bottles!
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Catalog Number: EC-875
National Diagnostics’ Denaturation Solution is a ready-to-use buffer solution of sodium chloride and sodium hydroxide in distilled/deionized water. It is especially designed for use in Southern and Northern Blotting, and in situ hybridization procedures.
Denaturation Solution contains 1.5M sodium chloride, 0.5M sodium hydroxide.
Quantity discount when you buy 4 or more bottles!
Catalog Number: EC-876
ational Diagnostics’ Neutralization Solution is a ready-to-use sodium chloride-tris buffer solution. It is especially designed for use in Southern and Northern Blotting, and in situ hybridization procedures.
Neutralization Solution contains 3M NaCl and 0.5M Tris in distilled/deionized water.
Catalog Number: EC-728
Size: 450 ml
Size: 450 ml
- Ideal for Western blotting membranes
- Premixed formula saves time, money, and effort
- Stain up to 90 mini blots
Catalog number: EC-878
Size: 1 Liter
Size: 1 Liter
- Blot in half the time versus conventional electroblotting buffers
- Very high-efficiency transfer
- Optimal pH; less harsh on proteins
Catalog number: EC-873
SSC Buffer (20X) is a concentrated solution of sodium chloride-sodium citrate in distilled/deionized water. SSC Buffer is widely used in nucleic acid blotting and hybridization protocols.
SSC Buffer (20X) is already in solution, which eliminates the weighing, mixing, and adjusting of pH necessary with powdered buffers. The 20X formulation allows for easy dilutions to all needed concentrations.
SSC Buffer (20X) contains 3M sodium chloride and 0.3M sodium citrate (pH 7.0).
Catalog number: EC-881
Size: 1 Liter
- Western Blot wash and incubation buffer
- Ultra-pure reagents in 18 Megohm water
- 0.2 Micron filtered and autoclaved
Catalog number: EC-882
Size: 1 Liter
- Western Blot wash and incubation buffer
- Ultra-pure reagents in 18 MegOhm water
- 0.2 micron filtered and autoclaved
Catalog number: EC-862
Size: 25 ml
Size: 25 ml
TE Buffer is an autoclaved 100X concentrated solution of 1 M tris-HCl, pH 8, with 0.1 M Na2EDTA. This buffer is the standard for DNA and RNA purification, processing and storage. National Diagnostics’ TE buffer is produced to the same exacting standards that have made all our buffers trusted by molecular biologists worldwide
Quantity discount when you buy 4 or more 4 liter bottles!
Catalog number: EC-880
- Manufactured with Ultra-Pure Electrophoresis Grade Reagents
- 18 MegOhm Water & 0.2 Micron Filtration
- Saves Time and Improves Reproducibility in Western Blotting
- Using PAGE to Determine Nucleic Acid Molecular Weight
- SSCP Analysis
- Sanger Sequencing
- Sample Preparation for Native PAGE of DNA
- Sample Prep for Denaturing PAGE of DNA
- S1 Mapping
- Run Conditions in Denaturing PAGE
- RNA Mapping
- RNA Electrophoresis
- Ribonuclease Protection
- Restriction Digest Mapping
- Primer Extension
- Preparing Denaturing DNA & RNA Gels
- Preparation of Denaturing Agarose Gels
- Preparation of Agarose Gels
- Pouring Sequencing Gels
- PFGE and FIGE
- PCR Analysis: Yield and Kinetics
- PCR Analysis: An Examination
- Native PAGE of DNA
- Mobility Shift Assay
- Methylation & Uracil Interference Assays
- Maxam & Gilbert Sequencing
- Manual Sequencing
- In Gel Enzyme Reactions
- Heteroduplex Analysis
- Gel Preparation for Native PAGE of DNA
- Gel Electrophoresis of PCR Products
- DNase I Footprinting
- DNA/RNA Purification from PAGE Gels
- DNA/RNA Purification from Agarose Gels – Electroelution
- Differential Display
- Denaturing Polyacrylamide Gel Electrophoresis of DNA & RNA
- Conformational Analysis
- Automated Sequencers
- Analysis of DNA/Protein Interactions
- Agarose Gel Electrophoresis of DNA and RNA – Uses and Variations
- Agarose Gel Electrophoresis of DNA and RNA – An Introduction