Fundamentals of Liquid Scintillation Counting ARTICLES
Here are a few guidelines which you can use to process fixed tissue using Histo-Clear and Histo-Clear II: Histo-Clear Automated Tissue Processing Schedule Process Bath 18 Hour Cycle (Time in Hours) 24 Hour Cycle (Time…
Particulate in the sample: Standard loading buffers are often unable to completely dissolve complex samples- fragments of bacterial cell walls, for example, can be carried over into the well. Particulate material in the well blocks…
Blotches can be caused by overhandling of the gel, or by handling the gel without gloves. This can create uneven surfaces or protein deposits that can bind Coomassie to the gel. To minimize this problem,…
Uneven staining is almost always the result of insufficient agitation during staining. The gel is initially more dense than the staining solution and will tend to sink to the bottom of the dish. Some portions…
A high background which obscures the bands, or in combination with fainter than usual bands, indicates dye binding to the gel matrix, or contamination of the matrix with a dye-binding material (most often a protein).…
SDS in the staining solution: Most users save and re-use their staining solutions. This is fine for a couple of cycles, but over time enough SDS elutes from the gels to accumulate to significant levels…
If your gel doesn’t look like this one, click on the problem below to find the solution: Faint bands on a low background Learn More Faint bands on a high background Learn More Uneven Staining…
The OptiClear Solvents OptiClear OE-101 OptiClear R OE-102 OptiClear E OE-104 OptiClear S OE-105 OptiClear S2 OE-107 OptiClear W OE-106 Flash Point (oF) 120 142 45 147 177 199 Non-Toxic? Yes Yes Yes Yes Yes…
Prior to the introduction of liquid scintillation counting, a primary route of radiotracer analysis was to combust the organic material and detect the 14CO2 so generated in a gas phase proportional counter. Many protocols still…
Most research applications of radioisotopes require an eventual quantitation of the isotope, which is done by measuring the intensity of radiation emitted. Common nomenclature expresses this intensity as disintegrations per minute (DPM). The becquerel (Bq)…
- Waste Disposal Issues in Scintillation Counting
- The Complete Scintillation Cocktail
- Radioactive Emissions and the Use of Isotopes in Research
- Preparing Tissue Samples for Scintillation Counting
- Preparing Samples in PAGE Gels for LSC
- Mechanism of Liquid Scintillation Counting
- Measurement of Radiation and Isotope Quantitation
- Liquid Scintillation Signal Interpretation
- Liquid Scintillation and Radiation Safety
- HPLC Flow Counting
- Counting Samples on Cellulose-Ester Filters
- Counting Samples from TLC Plates by LSC
- Counting Efficiency and Quenching
- Counting Carbon Dioxide by LSC
- Chemiluminescence and Static Electricity
- Assaying Discrete Samples by Liquid Scintillation Counting