ProtoBlock System
$68.00
Catalog Number: CL-252
- Protein Blocking Solution for Immunoassays
- Eliminates Endogenous Background
Description
Catalog Number: CL-252
- Protein Blocking Solution for Immunoassays
- Eliminates Endogenous Background
Western Blot immunoassay is a highly specific method of protein analysis using affinity-purified antibodies. An enzyme-linked affinity-purified antibody used in conjunction with a highly sensitive chemiluminescent substrate provides an excellent method for detection and characterization of samples bound to membranes through Western Blot or Dot ELISA. Following transfer of proteins to a membrane, it is very important to block the membrane with a good blocking solution. ProtoBlock Solution is designed to work optimally with Horseradish Peroxidase Luminescent Kit (ProtoGlow ECL kit, order number CL-300). ProtoBlock solution contains a broad spectrum of proteins, protein analogs, detergents, and buffers which are designed to minimize endogenous backgrounds. ProtoBlock solution may be used for Western Blotting, Southern Blotting, immunoassays, and in situ hybridization.
Storage: All system components may be stored at room temperature (20°C).
Additional information
| Weight | 2 lbs |
|---|---|
| Dimensions | 8 × 3 × 10 in |
Protocol
ProtoBlock System Protocol
The following reagents are supplied with this system:
- ProtoBlock Reagent A (100 grams)
- ProtoBlock Reagent B (200 ml)
To prepare 200 ml of ProtoBlock solution, add 10 grams of ProtoBlock Reagent A to 170 ml of deionized water. Mix well and add 20 ml of ProtoBlock Reagent B. This solution is used to block the membrane after protein transfer and to dilute the antibodies used in the immunoassays.
If any blocking solution remains, store at 4oC for future use. Reconstituted solution is stable at 4-8oC for one week.
Catalog Number: CL-252 View/Download Full Protocol >
Safety Overview
ProtoBlock System Reagent A Safety Overview
Safety Summary (see MSDS for complete information before using the product):
Appearance and Odor
White powder
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
As part of good industrial and personal hygiene and safety procedure, avoid all unnecessary exposure to the chemical substance and ensure prompt removal from skin, eyes, and clothing.
As part of good industrial and personal hygiene and safety procedure, avoid all unnecessary exposure to the chemical substance and ensure prompt removal from skin, eyes, and clothing.
ProtoBlock System Reagent B Safety Overview
Safety Summary (see MSDS for complete information before using the product):
Appearance and Odor
Colorless liquid
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
Contains 0.1% of a preservative which is harmful if swallowed, may be absorbed through skin. May cause allergenic reaction in susceptible individuals.
Contains 0.1% of a preservative which is harmful if swallowed, may be absorbed through skin. May cause allergenic reaction in susceptible individuals.
Full SDS
ProtoBlock System – Reagent A
ProtoBlock System – Reagent A
