Oxygen Assays Articles
In at least one important way, measuring hydrogen peroxide is substantially easier than measuring superoxide. Superoxide is unstable in aqueous solution—its steady state concentration cannot be measured directly. As a result, superoxide “levels” must be…
Hydrogen peroxide, like superoxide, can react with a variety of targets in the cell, and has been associated with a number of diseases. Aerobic organisms express catalase and peroxidase enzymes to prevent damage by H2O2.…
Overview Superoxide has been implicated in diseases ranging from Alzheimer’s to diabetes. The wide range of pathologies associated with superoxide is the result of its ability to react with a variety of cellular targets, including…
Oxygen is used by a great variety of organisms as a means for producing energy. The redox potential of the oxygen-water couple is 1.229 Volts, meaning that a relatively large amount of energy is released…
Chromogenic and luminometric substances are also available for horseradish peroxidase (HRP). The “classic” chromogen used with HRP is diaminobenzidine (DAB). In the presence of H2O2, HRP will oxidize DAB, creating a water insoluble brown precipitate.…
- Working Safely with Fixatives
- The Chemistry of Dyes and Staining
- Suggested procedures for processing fixed tissue
- Staining Procedures
- Sectioning
- Overview of the Paraffin Technique
- Overview of Fixation
- Non-Aldehyde Fixatives
- Mounting Tissue Sections
- Factors Affecting Fixation
- Embedding
- Dehydration
- Decalcifying Tissue for Histological Processing
- Clearing Tissue Sections
- Artifacts in Histologic Sections
- Aldehyde Fixatives