Histology
Calci-Clear
$36.00 – $430.00
Hazardous Shipping Fees: This product may incur an additional hazardous shipping fee. We will pack the products to minimize all shipping costs, and the final charge will reflect the shipping cost and any applicable hazardous fees charged by FedEx.
Catalog number: HS-104
- Moderate Acidity Decalcifier
- Mildness Balanced with Performance
Description
Hazardous Shipping Fees: This product may incur an additional hazardous shipping fee. We will pack the products to minimize all shipping costs, and the final charge will reflect the shipping cost and any applicable hazardous fees charged by FedEx.
Catalog number: HS-104
- Moderate Acidity Decalcifier
- Mildness Balanced with Performance
Calci-Clear may be used to decalcify any tissue type. This solution of efficient chelating and sequestering agents removes and binds all calcium and other metal ions under treatment. Interference with subsequent staining is reduced while tissue distortion and structure loss is minimized.
Additional information
| Weight | 9 lbs |
|---|---|
| Dimensions | 7 × 5 × 12 in |
Protocol
Procedure
- Rinse fixed tissue in running water for five minutes.
- Submerge the tissue sample in a volume of Calci-Clear equal to at least ten times the volume of the sample. Take care that the Calci-Clear has free access to all surfaces of the tissue.
- Check tissue every thirty to sixty minutes. Although some workers consider decalcification complete when the tissue begins to float, for some types of tissue this is not an accurate indicator of the endpoint of decalcification. A chemical test may be employed to more accurately confirm the endpoint of decalcification.
- Changing the solution is recommended every two or three hours.
Safety Overview
Safety Summary (see SDS for complete information before using product):
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
DANGER! CORROSIVE. LIQUID AND MIST CAUSE SEVERE BURNS TO ALL BODY TISSUE. MAY BE FATAL IF SWALLOWED. HARMFUL IF INHALED. INHALATION MAY CAUSE LUNG DAMAGE. VAPOR IS IRRITATING TO EYES AND RESPIRATORY TRACT. FLAMMABLE LIQUID AND VAPOR. Health hazards given on this data sheet apply to concentrated solutions of acid. Hazards of dilute solutions may be reduced, depending upon the concentration. Degree of hazard for these reduced concentrations is not currently addressed in the available literature
- Working Safely with Fixatives
- Tissue Processing for Electron Microscopy
- The Chemistry of Dyes and Staining
- Suggested procedures for processing fixed tissue
- Staining Tissue Sections for Electron Microscopy
- Staining Procedures
- Sectioning Tissue for Electron Microscopy
- Sectioning
- Overview of the Paraffin Technique
- Overview of Fixation
- Non-Aldehyde Fixatives
- Mounting Tissue Sections
- Immunohistochemistry
- Fixing Tissue for Electron Microscopy
- Factors Affecting Fixation
- Embedding
- Electron Microscopy
- Detection Systems in Immunohistochemistry
- Dehydration
- Decalcifying Tissue for Histological Processing
- Clearing Tissue Sections
- Artifacts in Histologic Sections
- Antibody Binding
- Aldehyde Fixatives
