Electrophoresis
Glass Free
$147.00
Hazardous Shipping Fees: This product may incur an additional hazardous shipping fee. We will pack the products to minimize all shipping costs, and the final charge will reflect the shipping cost and any applicable hazardous fees charged by FedEx.
Catalog Number: EC-621
Size: 450 ml
- Coats glass casting plates for easy release
- Prevents gel from binding to the upper casting plate
Description
Hazardous Shipping Fees: This product may incur an additional hazardous shipping fee. We will pack the products to minimize all shipping costs, and the final charge will reflect the shipping cost and any applicable hazardous fees charged by FedEx.
Catalog Number: EC-621
Size: 450 ml
Hazardous Shipping Fee
- Coats glass casting plates for easy release
- Prevents gel from binding to the upper casting plate
Glass Free from National Diagnostics coats glass casting plates for easy release of polyacrylamide gels. Glass Free prevents the cast gel from binding to the removable upper glass casting plate. Glass Free, used in conjunction with Glass Bond, guarantees whole, manageable electrophoretic gels time after time. Glass Free is reusable and has a shelf life of two years.
Storage: Glass Free is best stored tightly capped in a cool dry area.
Glass Free method of use:
- For preparing glass plates for gel casting, Glass Free is to be used only on the removable upper plate. Clean the glass with detergent, water, distilled water, and methanol. If the glass plate has previously been treated with Glass Bond, it is first necessary to soak the glass in 20% sodium hydroxide for 3-16 hours and then wash with water and distilled water.
- After the plate is thoroughly dry, immerse it in a dish of Glass Free for 5 minutes, making sure that all areas of the glass are well covered by the reagent. Remove any trapped air bubbles. Always work with Glass Free in a hood. Use latex, PVC or polyethylene gloves.
- Remove the glass plate from the Glass Free vessel and thoroughly rinse it with an organic solvent such as toluene, xylene, or mineral spirits.
- Rinse the solvent off with methanol. Lightly buff out the methanol with a paper towel. The plates are now ready for use. Store them in a plastic bag interleaved with paper.
- To reuse a plate that has previously been treated with Glass Free, wash the plate with water and distilled water and follow by a final rinse with methanol and buff.
Additional information
| Weight | 2.5 lbs |
|---|---|
| Dimensions | 7 × 7 × 11 in |
Protocol
Glass Free Method of Use
Basic Protocol
WARNING: Always work with Glass Free in a hood
- For preparing glass plates for gel casting, Glass Free is to be used only on the removable upper plate. Clean the glass with detergent, water, distilled water, and methanol.
- After it is thoroughly dry immerse the plate in a dish of Glass Free for 5 minutes, making sure that all areas of the glass are well covered by the reagent. Remove any trapped air bubbles. Use latex, PVC or polyethylene gloves.
- Remove the glass plate from the Glass Free vessel and thoroughly rinse it with an organic solvent such as toluene, xylene or mineral spirits.
- Rinse the solvent off with methanol. Lightly buff out the methanol with a paper towel. The plates are now ready for use. Store them in a plastic bag and interleaved with paper.
Note: To reuse a plate that has previously been treated with Glass Free wash the plate with water and distilled water, and follow by a final rinse with methanol and buff.
Safety Overview
Safety Summary (see SDS for complete information before using product):
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Appearance and Odor:
Clear colorless liquid, irritating fumes.
Highly flammable. Irritating to eyes, respiratory system and skin.
Do not breathe fumes. If swallowed, do not induce vomiting. Seek medical advice immediately and show this container or label
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EMERGENCY OVERVIEW – IMMEDIATE HAZARDDANGER! EXTREMELY FLAMMABLE LIQUID AND VAPOR. VAPOR MAY CAUSE FLASH FIRE. WATER REACTIVE. CORROSIVE. MAY BE FATAL IF SWALLOWED. HARMFUL IF INHALED OR ABSORBED THROUGH SKIN. MATERIAL IS EXTREMELY DESTRUCTIVE TO THE UPPER RESPIRATORY TRACT, EYES, AND SKIN. CAUSES SEVERE BURNS TO EVERY AREA OF CONTACT.
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- UV Shadowing
- Using PAGE to Determine Nucleic Acid Molecular Weight
- Uneven Staining
- The Polyacrylamide Matrix-Buffer Strength
- The Polyacrylamide Matrix
- The Mechanical and Electrical Dynamics of Gel Electrophoresis — Electrophoresis System Dynamics
- The Mechanical and Electrical Dynamics of Gel Electrophoresis – Ohm’s Law
- The Mechanical and Electrical Dynamics of Gel Electrophoresis – Intro and Sample Mobility
- The Electrophoresis Matrix
- The Agarose Matrix
- Staining Proteins Immobilized on Membranes
- Staining Protein Gels with Coomassie Blue
- SSCP Analysis
- Southern Blotting
- Smeared Bands
- Silver Staining Protein Gels
- Silver Staining DNA Gels
- Sanger Sequencing
- Sample Preparation for SDS-PAGE
- Sample Preparation for Native Protein Electrophoresis
- Sample Preparation for Native PAGE of DNA
- Sample Prep for Denaturing PAGE of DNA
- S1 Mapping
- Run Conditions in Denaturing PAGE
- RNA Mapping
- RNA Electrophoresis
- Ribonuclease Protection
- Restriction Digest Mapping
- Radioactive Emissions and the Use of Isotopes in Research
- Protein Fixation on Gels
- Primer Extension
- Preparing Denaturing DNA & RNA Gels
- Preparation of Denaturing Agarose Gels
- Preparation of Agarose Gels
- Pouring Sequencing Gels
- Post-Electrophoretic Visualization with Nuclistain
- PFGE and FIGE
- Peptide Mapping
- PCR Analysis: Yield and Kinetics
- PCR Analysis: An Examination
- Overview of Western Blotting
- Northern Blotting
- Native Protein Electrophoresis
- Native PAGE of DNA
- Multiphasic Buffer Systems
- Mobility Shift Assay
- Methylation & Uracil Interference Assays
- Method for Western Blotting
- Mechanism of Immunostaining
- Mechanism of Immunostaining
- Measuring Molecular Weight with SDS-PAGE
- Maxam & Gilbert Sequencing
- Manual Sequencing
- Isotachophoresis
- Isoelectric Focusing
- In Gel Enzyme Reactions
- Immunostaining with Alkaline Phosphatase
- Immuno-Electrophoresis / Immuno-Diffusion
- Horizontal and Vertical Gel Systems – Vertical Tube Gels
- Horizontal and Vertical Gel Systems – The Vertical Slab Gel System
- Horizontal and Vertical Gel Systems – The Horizontal Gel System
- Homogeneous Buffer Systems
- Heteroduplex Analysis
- Guide Strip Technique
- Gel Preparation for Native Protein Electrophoresis
- Gel Preparation for Native PAGE of DNA
- Gel Electrophoresis of RNA & Post Electrophoretic Analysis
- Gel Electrophoresis of PCR Products
- Faint bands, low background
- Faint Bands, High Background
- Ethidium Bromide Staining
- Enzyme Linked Immunosorbent Assay (ELISA)
- Electrophoresis Buffers-Choosing the Right Buffer
- Electrophoresis Buffers–The Henderson-Hasselbalch Equation
- DNase I Footprinting
- DNA/RNA Purification from PAGE Gels
- DNA/RNA Purification from Agarose Gels – Electroelution
- Differential Display
- Denaturing Protein Electrophoresis: SDS-PAGE
- Denaturing Polyacrylamide Gel Electrophoresis of DNA & RNA
- Coomassie Blue Stain- Troubleshooting
- Conformational Analysis
- Casting Gradient Gels
- Buffer Additives-Surfactants
- Buffer Additives-Reducing Agents
- Buffer Additives-Hydrogen Bonding Agents
- Blotches on Gel
- Biological Macromolecules: Nucleic Acids
- Biological Macromolecules – Proteins
- Autoradiography
- Autoradiographic Enhancement with Autofluor
- Automated Sequencers
- Analysis of DNA/Protein Interactions
- An Overview of Northern and Southern Blotting
- Alkaline Blotting
- Agarose Gel Electrophoresis of DNA and RNA – Uses and Variations
- Agarose Gel Electrophoresis of DNA and RNA – An Introduction
- Activity Stains
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