Oxygen Assays
Hydrogen Peroxide Assay Kit
Catalog Number: CL-204
- Sensitive, Quantitative Assay for Hydrogen Peroxide
- Detects as Little as 15ng/ml
- Easy-to-use, Colorimetric System
Description
- Sensitive, Quantitative Assay for Hydrogen Peroxide
- Detects as Little as 15ng/ml
- Easy-to-use, Colorimetric System
Hydrogen peroxide is a part of the oxygen reduction pathway, produced by the two-electron reduction of molecular oxygen, or by the one-electron reduction of superoxide anion radical. Hydrogen peroxide is a potent oxidant, and the levels of hydrogen peroxide must be accurately determined in order to fully characterize the oxidative state of the system under study.
National Diagnostics Hydrogen Peroxide Assay Kit is a rapid, sensitive, and quantitative method for the determination of Hydrogen Peroxide in chemical or biological systems. The assay is based upon formation of a complex between xylenol orange and ferric iron, which is produced by the peroxide dependent oxidation of ferrous iron. This reaction is quantified colorimetrically, detecting as little as 15ng/ml of peroxide. Linear results can be obtained up to approximately 1000ng/ml.
National Diagnostics Hydrogen Peroxide Assay is the ideal complement to our well established Diogenes Superoxide detection system. Using these kits in tandem, researchers can now quantitatively determine the first two steps in the oxygen reduction pathway.
Additional information
| Weight | 2 lbs |
|---|---|
| Dimensions | 8 × 3 × 10 in |
Protocol
Hydrogen Peroxide Assay Protocol
Assay Reagent Preparation
Prepare 1.8ml of reagent per sample to run tests in duplicate. To prepare 20 ml combine 19.8 ml of Component A with 0.2ml Component B. The mixture is stable at room temperature for one working day.
Assay Procedure
- Mix 0.9 ml of Assay Reagent with up to 0.1 ml of sample.
- Incubate at room temperature for at least 30 minutes to allow for complete color development.
- Read absorbance at 560nm.
Safety Overview
Hydrogen Peroxide Assay Reagent A Safety Overview
Safety Summary (see SDS for complete information before using the product):
Appearance and Odor
Dark, straw-colored liquid.
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
SORBITOL
HIGH CONCENTRATIONS MAY IRRITATE EYES AND RESPIRATORY TRACT.
XYLENOL ORANGE
Hazardous in case of skin contact (irritant), of eye contact (irritant), of ingestion, of inhalation.
EMERGENCY OVERVIEW – CHRONIC HAZARD WARNING:
SORBITOL
AS PART OF GOOD LABORATORY AND PERSONAL HYGIENE AND SAFETY PROCEDURE, AVOID ALL UNNECESSARY EXPOSURE TO THE CHEMICAL SUBSTANCE AND ENSURE PROMPT REMOVAL FROM SKIN, EYES, AND CLOTHING.
XYLENOL ORANGE
Repeated or prolonged exposure is not known to aggravate medical condition.
Hydrogen Peroxide Assay Reagent B Safety Overview
Safety Summary (see MSDS for complete information before using product):
Toxic by inhalation, in contact with skin and if swallowed.
Avoid contact with skin and eyes.
TOXIC BY INHALATION, IN CONTACT WITH SKIN AND IF SWALLOWED.
Full MSDS
Hydrogen Peroxide Assay Kit – Reagent A
Hydrogen Peroxide Assay Kit – Reagent A
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