Nuclistain

• UV Free Visualization of DNA and RNA
• Improves Yield in PCR Purification from Agarose Gels
• Easy and Safe

Nuclistain improves the yield of smaller and medium sized DNA (<500 bp) obtained by purification from agarose. One gel was stained with Nuclistain and the other with EtBr. The DNA was subsequently purified from each and then run side by side on a third gel, visualized by EtBr. The results show the improved yield from the Nuclistain.

Nuclistain is intended as a replacement for the conventionally used ethidium bromide for the rapid detection of double and single-stranded DNA and RNA in agarose and polyacrylamide gels. Its sensitivity is nearly comparable, with the capability of detecting as little as 10-50 ng of nucleic acid sample. The advantage of Nuclistain is that the results are visible under normal lighting conditions, eliminating the possibility of sample damage by UV radiation. Furthermore, Nuclistain visualization dramatically improves the yield of smaller and medium-sized DNA (<500 bp) obtained by purification from agarose. Nuclistain binding is reversible and does not modify nucleic acids.

Nuclistain is extremely easy to use. One 25 ml bottle of Nuclistain will make 2.5 liters of stain solution. The separated DNA appears as dark blue bands on a light blue background.