ProtoBlock System

The following reagents are supplied with this system:

  • ProtoBlock Reagent A (100 grams)
  • ProtoBlock Reagent B (200 ml)

To prepare 200 ml of ProtoBlock solution, add 10 grams of ProtoBlock Reagent A to 170 ml of deionized water.  Mix well and add 20 ml of ProtoBlock Reagent B.  This solution is used to block the membrane after protein transfer and to dilute the antibodies used in the immunoassays.

If any blocking solution remains, store at 4oC for future use.  Reconstituted solution is stable at 4-8oC for one week.

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