Version 3.0
Date revised: 4/26/2019
SAFETY DATA SHEET
Conforms to regulation (EC) no. EU 453/2010
SECTION 1 - IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING
1.1 Product Identifier
1.2 Relevant Identified Uses of the Substance/Mixture and Uses Advised Against
Investigational research by professional users
1.3 Details of the Supplier of the Safety Data Sheet
| Manufacturer National Diagnostics 305 Patton Drive Atlanta, GA 30036 (404) 699-2121 (800) 526-3867 info@nationaldiagnostics.com |
1.4 Emergency Telephone Number
ChemTel Inc.
Contract number MIS8894340
1-800 255-3924 (United States, Canada, Puerto Rico & US Virgin Islands)
01-800-099-0731 (Mexico)
400-120-0751 (China)
000-800-100-4086 (India)
1-300-954-583 (Australia)
0-800-591-6042 (Brazil)
+1-813-255-3924 (All other regions)
SECTION 2 - HAZARDS IDENTIFICATION
2.1 Classification of the Substance or Mixture
Not a hazardous substance or mixture according to regulation (EC) No. 1272/2008.
2.2 Label Elements
This product has no labeling elements associated with EC directives or respective national laws.
2.3 Other Hazards
None found.
SECTION 3 - COMPOSITION/INFORMATION ON INGREDIENTS
3.2 Mixture
Chemical Names/Description
Alkaline formamide loading buffer
Component List
| Component | % Comp. | CAS # | EC # | 1278/2008 Classification |
|---|---|---|---|---|
| Formamide | 95 | 75-12-7 | 200-842-0 | H351, H360 |
| Water | 5 | 7732-18-5 | 231-791-2 | N.A. |
| Bromophenol Blue | <1 | 115-39-9 | 204-086-2 | N.A. |
| Sodium Hydroxide | <1 | 1310-73-2 | 215-185-5 | H290, H314 |
| Xylene Cyanol | <1 | 2650-17-1 | 220-167-5 | H315, H319, H335 |
SECTION 4 - FIRST AID MEASURES
4.1 Description of First Aid Measures
Inhalation
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician.
Ingestion
DO NOT INDUCE VOMITING. If swallowed and the person is conscious, immediately give large amounts of water. Get medical attention.
Skin
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.
Eyes
Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally. Get medical attention immediately.
4.2 Most Important Symptoms and Effects, Both Acute and Delayed
Inhalation
Formamide:
Symptoms may include coughing, shortness of breath. Excessive inhalation of vapor may cause symptoms that parallel ingestion, ranging from headache to unconsciousness, depending upon the duration and level of the exposure.
Water:
No information found.
Bromophenol Blue:
Coughing and shortness of breath.
Sodium Hydroxide:
Sneezing, sore throat or runny nose.
Xylene Cyanol:
Coughing, sore throat.
Ingestion
Formamide:
May cause headache, dizziness, nausea, vomiting, abdominal pain, and unconsciousness. May affect the repoductive system.
Water:
No information found.
Bromophenol Blue:
Effects not determined.
Sodium Hydroxide:
Bleeding, vomiting, diarrhea, fall in blood pressure. Symptoms may appear days after exposure.
Xylene Cyanol:
None identified.
Skin
Formamide:
Symptoms include redness, itching, and pain. May be absorbed through the skin with symptoms parallel to ingestion.
Water:
No information found.
Bromophenol Blue:
Redness and pain.
Sodium Hydroxide:
Redness, pain, burns.
Xylene Cyanol:
Pain and redness.
Eyes
Formamide:
Redness and pain.
Water:
No information found.
Bromophenol Blue:
Redness and pain.
Sodium Hydroxide:
Redness, pain, tearing.
Xylene Cyanol:
Pain and redness.
4.3 Indication of Any Immediate Medical Attention and Special Treatment Needed
Unknown/not applicable
SECTION 5 - FIRE FIGHTING MEASURES
5.1 Extinguishing media
Use media appropriate to the primary cause of fire.
5.2 Special Hazards Arising from the Substance/Mixture
Hazardous Combustion Products
N.A.
Hazardous Decomposition Products
Sodium oxide. Decomposition by reaction with certain metals releases flammable and explosive hydrogen gas.
Hazardous Polymerization
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
5.3 Advice for Firefighters
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.
5.4 Further Information
No data available.
SECTION 6 - ACCIDENTAL RELEASE MEASURES
6.1 Personal Precautions
Wear appropriate protective equipment as specified in Section 8.
6.2 Environmental Precautions
Prevent discharge into the environment. Dike spills and stop leakage where practical. Do not allow material to enter drains.
6.3 Methods and Materials for Containment and Cleaning Up
Contain spills with absorbing material such as sawdust. Sweep and place in covered disposal container. Wash area with water.
6.4 References to Other Sections
For disposal information see Section 13. For protective clothing and equipment see Section 8.
SECTION 7 - HANDLING AND STORAGE
7.1 Precautions for Safe Handling
Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
7.2 Conditions for Safe Storage (including any incompatibles)
Keep in a tightly closed container, stored in a cooled, dry, ventilated area. Protect from physical damage. Isolate from incompatible materials (section 10).
Incompatibles
Formamide:
Acids, alkalines, iodine, pyridine, and sulfur trioxide. Copper, brass, lead, and rubber are attacked by formamide.
Water:
No incompatibility data found.
Bromophenol Blue:
Strong oxidizers.
Sodium Hydroxide:
Acids, aluminum, tin and zinc metals.
Xylene Cyanol:
Strong oxidizing agents, strong reducing agents.
7.3 Specific End Uses
Investigational research by professional users
SECTION 8 - EXPOSURE CONTROLS/PERSONAL PRECAUTIONS
8.1 Control Parameters
Component: Formamide
ACGIH Threshold Limit Value (TLV):
10 ppm TWA
OSHA Permissable Exposure Limit (PEL):
None established
Component: Water
ACGIH Threshold Limit Value (TLV):
None established
OSHA Permissable Exposure Limit (PEL):
None established
Component: Bromophenol Blue
ACGIH Threshold Limit Value (TLV):
none established
OSHA Permissable Exposure Limit (PEL):
None established
Component: Sodium Hydroxide
ACGIH Threshold Limit Value (TLV):
2 mg/m3 (TLV)
OSHA Permissable Exposure Limit (PEL):
2 mg/m3
Component: Xylene Cyanol
ACGIH Threshold Limit Value (TLV):
none established
OSHA Permissable Exposure Limit (PEL):
None established
8.2 Exposure Controls
Engineering Controls
A system of local and/or general exhaust is recommended to keep employee exposures below the Airborne Exposure Limits. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source.
Respiratory Protection
For conditions of use where exposure to the dust or mist is apparent, a full-face dust/mist respirator may be worn. For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-supplied respirator.
Eye Protection
Use chemical safety goggles and/or a full face shield where splashing is possible. Maintain eye wash fountain and quick-drench facilities in work area.
Skin Protection
Wear protective gloves and clean body covering clothing.
SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on Basic Physical & Chemical Properties
| a. Appearance | Clear colorless solu | b. Odor | None |
| c. Odor Threshold | N.A. | d. pH | 13 - 14 |
| e. Melting/Freezing Point (oC) | 2-3 | f. Boiling point (oC) | 210 |
| g. Flash Point (oC) | 154 | h. Evaporation Rate | N.A. |
| i. Flammability | N.A. | j. Upper/Lower Flammability or Explosive Limits | N.A. |
| k. Vapor Pressure | 1mbar @700 deg C | l. Vapor Density (Air = 1) | > 1.0 |
| m. Relative Density | 1.1300 g/cm3 | n. Water Solubility | 1 g/ 0.9 ml water |
| o. Partition Coefficient n-octanol/water | Mixture | p. Autoignition Temperature (oC) | N.A. |
| q. Decomposition Temperature (oC) | Not applicable. | r. Viscosity | No data available. |
| s. Explosive Properties | N.A. | t. Oxidizing Properties | N.A. |
SECTION 10 - STABILITY AND REACTIVITY
10.1 Reactivity
10.2 Chemical Stability
Stable under ordinary conditions of use and storage. Very hygroscopic. Can slowly pick up moisture from air and react with carbon dioxide from air to form sodium carbonate.
10.3 Possibility of Hazardous Reactions
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
10.4 Conditions to Avoid
Moisture, dusting, and incompatibles.
10.5 Incompatible Materials
Formamide:
Acids, alkalines, iodine, pyridine, and sulfur trioxide. Copper, brass, lead, and rubber are attacked by formamide.
Water:
No incompatibility data found.
Bromophenol Blue:
Strong oxidizers.
Sodium Hydroxide:
Acids, aluminum, tin and zinc metals.
Xylene Cyanol:
Strong oxidizing agents, strong reducing agents.
10.6 Hazardous Decomposition Products
Sodium oxide. Decomposition by reaction with certain metals releases flammable and explosive hydrogen gas.
SECTION 11 - TOXICOLOGICAL INFORMATION
Product LD50 Values
Oral Rat LD50 (mg/kg)
Dermal Rabbit LD50 (mg/kg)
50000
Component Cancer List Status
| NTP Carcinogen | |||
| Known | Anticipated | IARC Category | |
|---|---|---|---|
| Formamide | No | No | None |
| Water | No | No | None |
| Bromophenol Blue | No | No | None |
| Sodium Hydroxide | No | No | None |
| Xylene Cyanol | No | No | None |
Potential Health Effects
Inhalation
Formamide
Causes irritation to the respiratory tract.
Water
No information found.
Bromophenol Blue
May cause irritation to the respiratory tract.
Sodium Hydroxide
Severe irritant. Effects from inhalation of mist vary from mild irritation to serious damage of the upper respiratory tract, depending on severity of exposure. Severe pneuomonitis may occur.
Xylene Cyanol
None identified.
Ingestion
Formamide
Causes irritation to the gastrointestinal tract. Affects the central nervous system. May affect the reproductive system.
Water
No information found.
Bromophenol Blue
Large oral doses may cause irritation to the gastrointestinal tract.
Sodium Hydroxide
Corrosive! Swallowing may cause severe burns of mouth, throat, and stomach. Severe scarring of tissue and death may result.
Xylene Cyanol
May be irritating to the mucous membranes and upper respiratory tract.
Skin
Formamide
Causes irritation to the skin. May be absorbed through the skin.
Water
No information found.
Bromophenol Blue
May cause irritation to the skin.
Sodium Hydroxide
Corrosive! Contact with skin may cause irritation or severe burns and scarring with greater exposures.
Xylene Cyanol
Irritation.
Eyes
Formamide
Causes irritation to the eyes.
Water
No information found.
Bromophenol Blue
May cause irritation to the eyes.
Sodium Hydroxide
Corrosive! Causes irritation of eyes, and with greater exposures it can cause burns that may result in permanent impairment of vision, even blindness.
Xylene Cyanol
Irritation.
Carcinogenicity
Formamide
Not listed as a known or anticipated carcinogen by NTP or IARC.
Water
No information available.
Bromophenol Blue
Not listed as a known or anticipated carcinogen by NTP or IARC.
Sodium Hydroxide
Not listed as a carcinogen by either NTP or IARC.
Xylene Cyanol
Not listed by NTP or IARC as a known or possible carcinogen.
Mutagenicity
Formamide
No information found.
Water
No information available.
Bromophenol Blue
No information found.
Sodium Hydroxide
No information found.
Xylene Cyanol
No information found.
Reproductive Toxicity
Formamide
May cause congenital malformation of the fetus.
Water
No information available.
Bromophenol Blue
No information found.
Sodium Hydroxide
No information found.
Xylene Cyanol
No information found.
Teratogenic Effects
Formamide
May cause congenital malformation of the fetus.
Water
No information available.
Bromophenol Blue
No information found.
Sodium Hydroxide
No information found.
Xylene Cyanol
No information found.
Routes of Entry
Formamide
Ingestion, inhalation, and absorption through the skin.
Water
No information available
Bromophenol Blue
No information found.
Sodium Hydroxide
Inhalation or ingestion.
Xylene Cyanol
No information found.
Target Organ Statement
Formamide
No information found.
Water
No information available
Bromophenol Blue
No information found.
Sodium Hydroxide
Persons with pre-existing skin disorders or eye problems or impaired respiratory function may be more susceptible to the effects of the substance.
Xylene Cyanol
No information found.
SECTION 12 - ECOLOGICAL INFOMATION
12.1 Toxicity
COMPONENT: Formamide
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | 6569 @ 96 hrs | >500 @ 48 hrs | >500 @ 96 hrs | >1000 @ 30min |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | no data | no data | no data | no data |
COMPONENT: Water
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: Bromophenol Blue
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: Sodium Hydroxide
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | LC50 (48hrs, golden orfe) 189mg/L | EC50 (48hr, daphnia) 40mg/L | No data | No data |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: Xylene Cyanol
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
12.2 Persistence and Degradability
Formamide
Readily biodegradable (99% degradation at 28 days)
Water
No data
Bromophenol Blue
No data
Sodium Hydroxide
No data
Xylene Cyanol
No data
12.3 Bioaccumulative Potential
Formamide
low expected bioaccumulation log BCF 0.5
Water
No data
Bromophenol Blue
No data
Sodium Hydroxide
No data
Xylene Cyanol
No data
12.4 Mobility in Soil
Formamide
log Koc: 0.93
Water
No data
Bromophenol Blue
No data
Sodium Hydroxide
No data
Xylene Cyanol
No data
12.5 Results of PBT and vPvB Assessment
Formamide
No Data
Water
No data
Bromophenol Blue
No data
Sodium Hydroxide
Mineral salt
Xylene Cyanol
No data
12.6 Other Adverse Effects
Formamide
No data
Water
None
Bromophenol Blue
None
Sodium Hydroxide
None
Xylene Cyanol
None
SECTION 13 - DISPOSAL CONSIDERATIONS
13.1 Waste Treatment Methods
Offer surplus or non-recyclable product to licensed disposal company. Disposal is subject to user compliance with applicable law and product characteristics at time of disposal. Dispose of packaging as product.
SECTION 14 - TRANSPORT INFORMATION
| ADR/RID | IATA | IMO | DOT | |
|---|---|---|---|---|
| 14.1 UN Number | N.A. | N.A. | N.A. | N.A. |
| 14.2 Shipping Name | Not regulated. | Not regulated. | Not regulated. | Not regulated. |
| 14.3 Hazard Class | N.A. | N.A. | N.A. | N.A. |
| 14.4 Packing Group | N.A. | N.A. | N.A. | N.A. |
| 14.5 Environmental Hazards | N.A. | N.A. | Not regulated | N.A. |
| 14.6 Special Precautions | N.A. | N.A. | N.A. | N.A. |
SECTION 15 - REGULATORY INFORMATION
15.1 Safety, Health and Environmental Regulations/Legislation Specific for the Substance/Mixture
United States
TSCA Regulatory Statement
All intentional ingredients are listed on the TSCA Inventory.
SARA 311/312 Hazard Categories
| Component | Fire | Pressure | Reactivity | Acute | Chronic |
|---|---|---|---|---|---|
| Formamide | No | No | No | Yes | Yes |
| Water | No | No | No | No | No |
| Bromophenol Blue | No | No | No | Yes | No |
| Sodium Hydroxide | No | No | Yes | Yes | No |
| Xylene Cyanol | No | No | No | Yes | Yes |
Europe
EEC Regulatory
All intentional ingredients are listed on the European EINECS Inventory.
SECTION 16 - OTHER INFORMATION
Revisional Updates
4/26/2019 - Updated Section 1.4
5/29/2015 - Updated Sections 2.1 and 3.2
4/16/2013 - Released Version 1.0
NFPA Codes
Health 3 Flammability 1 Reactivity 2
Dangers
Formamide
H351 - Suspected of causing cancer.
H360 - May damage fertility or the unborn child.
Water
None
Bromophenol Blue
None
Sodium Hydroxide
H290 - May be corrosive to metals.
H314 - Causes severe skin burns and eye damage.
Xylene Cyanol
H315 - Causes skin irritation.
H319 - Causes serious eye irritation.
H335 - May cause respiratory irritation.
MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without guarantee. Conditions of the use and suitability of the product for particular uses are beyond our control. All risks of use of the product are therefore assumed by the user. Nothing is intended as a recommendation for uses which infringe valid patents or as extending license under valid patents. Appropriate warnings and safe handling procedures should be provided to handlers and users.