Version 3.0
Date revised: 4/26/2019
SAFETY DATA SHEET
Conforms to regulation (EC) no. EU 453/2010
SECTION 1 - IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING
1.1 Product Identifier
1.2 Relevant Identified Uses of the Substance/Mixture and Uses Advised Against
Investigational research by professional users
1.3 Details of the Supplier of the Safety Data Sheet
| Manufacturer National Diagnostics 305 Patton Drive Atlanta, GA 30036 (404) 699-2121 (800) 526-3867 info@nationaldiagnostics.com |
1.4 Emergency Telephone Number
ChemTel Inc.
Contract number MIS8894340
1-800 255-3924 (United States, Canada, Puerto Rico & US Virgin Islands)
01-800-099-0731 (Mexico)
400-120-0751 (China)
000-800-100-4086 (India)
1-300-954-583 (Australia)
0-800-591-6042 (Brazil)
+1-813-255-3924 (All other regions)
SECTION 2 - HAZARDS IDENTIFICATION
2.1 Classification of the Substance or Mixture
Classification according to Regulation (EC) No. 1272/2008 [EU-GHS/CLP]
H351 - Carcinogenicity (Category 2)
H360 - Toxic to Reproduction (Category 1B)
2.2 Label Elements
| GHS Label Elements | ||
 | DANGER H351 - Suspected of causing cancer. | |
2.3 Other Hazards
None found.
SECTION 3 - COMPOSITION/INFORMATION ON INGREDIENTS
3.2 Mixture
Chemical Names/Description
Denaturing loading buffer containing formamide, EDTA and other components.
Component List
| Component | % Comp. | CAS # | EC # | 1278/2008 Classification |
|---|---|---|---|---|
| Formamide | 95 | 75-12-7 | 200-842-0 | H351, H360 |
| Ethylenediaminetetraacetic acid, disodium salt dihydrate | < 1 | 6381-92-6 | 205-358-3 | H332 |
SECTION 4 - FIRST AID MEASURES
4.1 Description of First Aid Measures
Inhalation
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician.
Ingestion
DO NOT INDUCE VOMITING. If swallowed and the person is conscious, immediately give large amounts of water. Get medical attention.
Skin
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.
Eyes
Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally. Get medical attention immediately.
4.2 Most Important Symptoms and Effects, Both Acute and Delayed
Inhalation
Formamide:
Symptoms may include coughing, shortness of breath. Excessive inhalation of vapor may cause symptoms that parallel ingestion, ranging from headache to unconsciousness, depending upon the duration and level of the exposure.
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Symptoms may include coughing and shortness of breath.
Ingestion
Formamide:
May cause headache, dizziness, nausea, vomiting, abdominal pain, and unconsciousness. May affect the repoductive system.
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Symptoms may include irritation and reddening to the mucous membranes of the mouth, esophagus, and gastrointestinal tract.
Skin
Formamide:
Symptoms include redness, itching, and pain. May be absorbed through the skin with symptoms parallel to ingestion.
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Symptoms may include irritation with redness and pain.
Eyes
Formamide:
Redness and pain.
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Symptoms may include irritation, redness, pain, and corneal damage.
4.3 Indication of Any Immediate Medical Attention and Special Treatment Needed
Unknown/not applicable
SECTION 5 - FIRE FIGHTING MEASURES
5.1 Extinguishing media
Water spray, dry chemical, alcohol-resistant foam, or carbon dioxide. Water or foam may cause frothing.
5.2 Special Hazards Arising from the Substance/Mixture
Hazardous Combustion Products
Highly toxic gases may be involved in fires of this product.
Hazardous Decomposition Products
Burning may produce ammonia, carbon monoxide, carbon dioxide, nitrogen oxides. At boiling point: ammonia, carbon monoxide, and hydrogen cyanide.
Hazardous Polymerization
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
5.3 Advice for Firefighters
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.
5.4 Further Information
No data available.
SECTION 6 - ACCIDENTAL RELEASE MEASURES
6.1 Personal Precautions
Wear appropriate protective equipment as specified in Section 8.
6.2 Environmental Precautions
Prevent discharge into the environment. Dike spills and stop leakage where practical. Do not allow material to enter drains.
6.3 Methods and Materials for Containment and Cleaning Up
Ventilate area. Cover with absorbent material (soda ash) to confine spill and sweep or shovel into container. Close container tightly. Avoid breathing vapors.
6.4 References to Other Sections
For disposal information see Section 13. For protective clothing and equipment see Section 8.
SECTION 7 - HANDLING AND STORAGE
7.1 Precautions for Safe Handling
Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling. Wear special protective equipment (Sec. 8) where exposures may exceed established levels.
7.2 Conditions for Safe Storage (including any incompatibles)
Keep frozen until ready for use.
Incompatibles
Formamide:
Acids, alkalines, iodine, pyridine, and sulfur trioxide. Copper, brass, lead, and rubber are attacked by formamide.
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Strong oxidizing agents, strong bases, aluminum, copper, copper alloys, nickel.
7.3 Specific End Uses
Investigational research by professional users
SECTION 8 - EXPOSURE CONTROLS/PERSONAL PRECAUTIONS
8.1 Control Parameters
Component: Formamide
ACGIH Threshold Limit Value (TLV):
10 ppm TWA
OSHA Permissable Exposure Limit (PEL):
None established
Component: Ethylenediaminetetraacetic acid, disodium salt dihydrate
ACGIH Threshold Limit Value (TLV):
None Established
OSHA Permissable Exposure Limit (PEL):
None Established
8.2 Exposure Controls
Engineering Controls
A system of local and/or general exhaust is recommended to keep employee exposures below the Airborne Exposure Limits. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source.
Respiratory Protection
If the exposure limit is exceeded, wear a supplied air, full-facepiece respirator, airlined hood, or full-facepiece self-contained breathing apparatus.
Eye Protection
Use chemical safety goggles and/or a full face shield where splashing is possible. Maintain eye wash fountain and quick-drench facilities in work area.
Skin Protection
Wear impervious protective clothing, including boots, gloves, lab coat, apron or coveralls, as appropriate, to prevent skin contact.
SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on Basic Physical & Chemical Properties
| a. Appearance | Blue solution | b. Odor | None |
| c. Odor Threshold | N.A. | d. pH | Neutral |
| e. Melting/Freezing Point (oC) | 2-3 | f. Boiling point (oC) | 210 |
| g. Flash Point (oC) | 154 | h. Evaporation Rate | < 1 (BuAc = 1) |
| i. Flammability | N.A. | j. Upper/Lower Flammability or Explosive Limits | No Data |
| k. Vapor Pressure | 1 @ 71C | l. Vapor Density (Air = 1) | 1.55 (air = 1) |
| m. Relative Density | 1.13 @20C | n. Water Solubility | Infinitely soluble |
| o. Partition Coefficient n-octanol/water | Mixture | p. Autoignition Temperature (oC) | No Data |
| q. Decomposition Temperature (oC) | Not applicable. | r. Viscosity | |
| s. Explosive Properties | N.A. | t. Oxidizing Properties | no oxidizing properties |
SECTION 10 - STABILITY AND REACTIVITY
10.1 Reactivity
Stable under normal conditions. Conditions to avoid: High temperatures. Materials to avoid: Oxidizing agents, Acids, Bases, iodine, pyridine, sulphur trioxide Corrodes metals: Aluminium, Iron, Copper, Corrodes natural rubber. Explosion and fire risk with particular chemicals: strong oxidizers, acids, bases Hazardous decomposition products: Carbon monoxide (CO), Hydrogen cyanide (hydrocyanic acid), Ammonia, nitrogen oxides (NOx)
10.2 Chemical Stability
Stable under ordinary conditions of use and storage. Absorbs moisture from the air.
10.3 Possibility of Hazardous Reactions
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
10.4 Conditions to Avoid
Heat, flames, ignition sources, and incompatibles.
10.5 Incompatible Materials
Formamide:
Acids, alkalines, iodine, pyridine, and sulfur trioxide. Copper, brass, lead, and rubber are attacked by formamide.
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Strong oxidizing agents, strong bases, aluminum, copper, copper alloys, nickel.
10.6 Hazardous Decomposition Products
Burning may produce ammonia, carbon monoxide, carbon dioxide, nitrogen oxides. At boiling point: ammonia, carbon monoxide, and hydrogen cyanide.
SECTION 11 - TOXICOLOGICAL INFORMATION
Product LD50 Values
Oral Rat LD50 (mg/kg)
5570
Dermal Rabbit LD50 (mg/kg)
No Data
Component Cancer List Status
| NTP Carcinogen | |||
| Known | Anticipated | IARC Category | |
|---|---|---|---|
| Formamide | No | No | None |
| Ethylenediaminetetraacetic acid, disodium salt dihydrate | No | No | None |
Potential Health Effects
Inhalation
Formamide
Causes irritation to the respiratory tract.
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Causes irritation of the mucous membrane and upper respiratory tract.
Ingestion
Formamide
Causes irritation to the gastrointestinal tract. Affects the central nervous system. May affect the reproductive system.
Ethylenediaminetetraacetic acid, disodium salt dihydrate
May cause gastrointestinal irritation with nausea, vomiting and diarrhea. Exposure may cause kidney injury, muscle cramps, bone-marrow depression, and a generalized allergic reaction.
Skin
Formamide
Causes irritation to the skin. May be absorbed through the skin.
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Causes skin irritation. Causes redness and pain.
Eyes
Formamide
Causes irritation to the eyes.
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Causes eye irritation. Causes redness and pain.
Carcinogenicity
Formamide
Not listed as a known or anticipated carcinogen by NTP or IARC.
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Not listed by NTP or IARC as a known or anticipated carcinogen.
Mutagenicity
Formamide
No information found.
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Cytogenetic Analysis: intraperitoneal - mouse 50mmol/L. DNA Inhibition: hamster fibroblast 500ug/L, rabbit kidney 250 umol/L.
Reproductive Toxicity
Formamide
May cause congenital malformation of the fetus.
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Fertility: Post-implantation mortality, oral - rat TDLo = 7632 mg/kg.
Teratogenic Effects
Formamide
May cause congenital malformation of the fetus.
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Embryo or Fetus: Stunted fetus, oral - rat TDLo = 7632 mg/kg. Specific developmental abnormalities: cardiovascular, craniofacial, musculoskeletal, respiratory, and urogenital, oral - rat TDLo = 7632 mg/kg.
Routes of Entry
Formamide
Ingestion, inhalation, and absorption through the skin.
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Inhalation, ingestion or skin contact.
Target Organ Statement
Formamide
No information found.
Ethylenediaminetetraacetic acid, disodium salt dihydrate
No information available
SECTION 12 - ECOLOGICAL INFOMATION
12.1 Toxicity
COMPONENT: Formamide
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | 6569 @ 96 hrs | >500 @ 48 hrs | >500 @ 96 hrs | >1000 @ 30min |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | no data | no data | no data | no data |
COMPONENT: Ethylenediaminetetraacetic acid, disodium salt dihydrate
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | LC50 (96hr, Lepomis macrochirus ):41mg/L | EC50 (485hr daphnia) 140mg/L | EC 50 (72hrs)>100mg/L | EC50>500mg/L |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | LOEC (Nicotiana tabacum ) 420mg/L | No data |
12.2 Persistence and Degradability
Formamide
Readily biodegradable (99% degradation at 28 days)
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Biodegradable (55% degradation in 20 days)
12.3 Bioaccumulative Potential
Formamide
low expected bioaccumulation log BCF 0.5
Ethylenediaminetetraacetic acid, disodium salt dihydrate
BCF 1.1-1.8
12.4 Mobility in Soil
Formamide
log Koc: 0.93
Ethylenediaminetetraacetic acid, disodium salt dihydrate
No data
12.5 Results of PBT and vPvB Assessment
Formamide
No Data
Ethylenediaminetetraacetic acid, disodium salt dihydrate
No data
12.6 Other Adverse Effects
Formamide
No data
Ethylenediaminetetraacetic acid, disodium salt dihydrate
None
SECTION 13 - DISPOSAL CONSIDERATIONS
13.1 Waste Treatment Methods
Offer surplus or non-recyclable product to licensed disposal company. Disposal is subject to user compliance with applicable law and product characteristics at time of disposal. Dispose of packaging as product.
SECTION 14 - TRANSPORT INFORMATION
| ADR/RID | IATA | IMO | DOT | |
|---|---|---|---|---|
| 14.1 UN Number | N.A. | N.A. | N.A. | N.A. |
| 14.2 Shipping Name | N.A. | N.A. | N.A. | N.A. |
| 14.3 Hazard Class | N.A. | N.A. | N.A. | N.A. |
| 14.4 Packing Group | N.A. | N.A. | N.A. | N.A. |
| 14.5 Environmental Hazards | N.A. | N.A. | Not regulated | N.A. |
| 14.6 Special Precautions | N.A. | N.A. | N.A. | N.A. |
SECTION 15 - REGULATORY INFORMATION
15.1 Safety, Health and Environmental Regulations/Legislation Specific for the Substance/Mixture
United States
TSCA Regulatory Statement
All intentional ingredients are listed on the TSCA
SARA 311/312 Hazard Categories
| Component | Fire | Pressure | Reactivity | Acute | Chronic |
|---|---|---|---|---|---|
| Formamide | No | No | No | Yes | Yes |
| Ethylenediaminetetraacetic acid, disodium salt dihydrate | No | No | No | Yes | No |
Europe
EEC Regulatory
All intentional ingredients are listed on the European EINECS Inventory.
SECTION 16 - OTHER INFORMATION
Revisional Updates
4/26/2019 - Updated Section 1.4
5/29/2015 - Updated Sections 2.1 and 3.2
6/27/2013- Released Version 1.0
NFPA Codes
Health 2 Flammability 1 Reactivity 0
Dangers
Formamide
H351 - Suspected of causing cancer.
H360 - May damage fertility or the unborn child.
Ethylenediaminetetraacetic acid, disodium salt dihydrate
H332 - Harmful if inhaled.
MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without guarantee. Conditions of the use and suitability of the product for particular uses are beyond our control. All risks of use of the product are therefore assumed by the user. Nothing is intended as a recommendation for uses which infringe valid patents or as extending license under valid patents. Appropriate warnings and safe handling procedures should be provided to handlers and users.