Version 3.0
Date revised: 4/26/2019
SAFETY DATA SHEET
Conforms to regulation (EC) no. EU 453/2010
SECTION 1 - IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING
1.1 Product Identifier
1.2 Relevant Identified Uses of the Substance/Mixture and Uses Advised Against
Investigational research by professional users
1.3 Details of the Supplier of the Safety Data Sheet
| Manufacturer National Diagnostics 305 Patton Drive Atlanta, GA 30036 (404) 699-2121 (800) 526-3867 info@nationaldiagnostics.com |
1.4 Emergency Telephone Number
ChemTel Inc.
Contract number MIS8894340
1-800 255-3924 (United States, Canada, Puerto Rico & US Virgin Islands)
01-800-099-0731 (Mexico)
400-120-0751 (China)
000-800-100-4086 (India)
1-300-954-583 (Australia)
0-800-591-6042 (Brazil)
+1-813-255-3924 (All other regions)
SECTION 2 - HAZARDS IDENTIFICATION
2.1 Classification of the Substance or Mixture
Classification according to Regulation (EC) No. 1272/2008 [EU-GHS/CLP]
H315 - Skin Corrosion/Irritation (Category 2)
H320 - Serious Eye Damage/Eye Irritation (Category 2B)
H335 - Specific Target Organ Toxicity, Single Exposure (Category 3)
2.2 Label Elements
| GHS Label Elements | ||
 | WARNING H315 - Causes skin irritation. | |
2.3 Other Hazards
None found.
SECTION 3 - COMPOSITION/INFORMATION ON INGREDIENTS
3.2 Mixture
Chemical Names/Description
Aqueous buffer
Component List
| Component | % Comp. | CAS # | EC # | 1278/2008 Classification |
|---|---|---|---|---|
| Tris-Base | 3 | 77-86-1 | 201-064-4 | H315, H319, H335 |
| Potassium Chloride | < 1 | 7447-40-7 | 231-211-8 | N.A. |
| Polysorbate 20 | < 1 | 9005-64-5 | 500-018-3 | N.A. |
| Water | 89 | 7732-18-5 | 231-791-2 | N.A. |
| Sodium Chloride | 8 | 7647-14-5 | 231-598-3 | N.A. |
SECTION 4 - FIRST AID MEASURES
4.1 Description of First Aid Measures
Inhalation
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician.
Ingestion
Induce vomiting immediately as directed by medical personnel. Never give anything by mouth to an unconscious person. Call a physician.
Skin
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.
Eyes
Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally. Get medical attention immediately.
4.2 Most Important Symptoms and Effects, Both Acute and Delayed
Inhalation
Tris-Base:
Coughing, shortness of breath.
Potassium Chloride:
Inhalations of high concentrations of dust may cause nasal or lung irritation.
Polysorbate 20:
Not expected to be a health hazard by inhalation.
Water:
No information found.
Sodium Chloride:
No information found.
Ingestion
Tris-Base:
Symptoms may include nausea, vomiting, and diarrhea. Large oral doses may cause weakness, collapse, blood clotting, and coma. The estimated lethal dose of Tris Base is 50 grams dry solid.
Potassium Chloride:
Large quantities can produce gastrointestinal irritation and vomiting.
Polysorbate 20:
Abdominal spasms, diarrhea.
Water:
No information found.
Sodium Chloride:
No information found.
Skin
Tris-Base:
Redness, itching, and pain.
Potassium Chloride:
May cause skin irritation.
Polysorbate 20:
Redness, swelling.
Water:
No information found.
Sodium Chloride:
No information found.
Eyes
Tris-Base:
Redness, itching, and pain.
Potassium Chloride:
Causes eye irritation. May cause chemical conjunctivitis.
Polysorbate 20:
Pain, redness.
Water:
No information found.
Sodium Chloride:
No information found.
4.3 Indication of Any Immediate Medical Attention and Special Treatment Needed
Unknown/not applicable
SECTION 5 - FIRE FIGHTING MEASURES
5.1 Extinguishing media
Water spray, dry chemical, alcohol-resistant foam, or carbon dioxide.
5.2 Special Hazards Arising from the Substance/Mixture
Hazardous Combustion Products
Highly toxic gases may be involved in fires of this product.
Hazardous Decomposition Products
Oxides of the contained metal and halogen, possibly also free, or ionic halogen
Hazardous Polymerization
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
5.3 Advice for Firefighters
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.
5.4 Further Information
No data available.
SECTION 6 - ACCIDENTAL RELEASE MEASURES
6.1 Personal Precautions
Wear appropriate protective equipment as specified in Section 8.
6.2 Environmental Precautions
Prevent discharge into the environment. Dike spills and stop leakage where practical. Do not allow material to enter drains.
6.3 Methods and Materials for Containment and Cleaning Up
Use absorbent material to collect and contain for salvage or disposal.
6.4 References to Other Sections
For disposal information see Section 13. For protective clothing and equipment see Section 8.
SECTION 7 - HANDLING AND STORAGE
7.1 Precautions for Safe Handling
Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
7.2 Conditions for Safe Storage (including any incompatibles)
Keep in a tightly closed container, stored in a cooled, dry, ventilated area.
Incompatibles
Tris-Base:
No incompatibility data found.
Potassium Chloride:
Bromine trifluoride; potassium permanganate plus sulfuric acid.
Polysorbate 20:
Strong oxidizers, acids.
Water:
No incompatibility data found.
Sodium Chloride:
No incompatibility data found.
7.3 Specific End Uses
Investigational research by professional users
SECTION 8 - EXPOSURE CONTROLS/PERSONAL PRECAUTIONS
8.1 Control Parameters
Component: Tris-Base
ACGIH Threshold Limit Value (TLV):
none established
OSHA Permissable Exposure Limit (PEL):
none established
Component: Potassium Chloride
ACGIH Threshold Limit Value (TLV):
10 mg/m3
OSHA Permissable Exposure Limit (PEL):
15 mg/m3
Component: Polysorbate 20
ACGIH Threshold Limit Value (TLV):
none established
OSHA Permissable Exposure Limit (PEL):
None established
Component: Water
ACGIH Threshold Limit Value (TLV):
None established
OSHA Permissable Exposure Limit (PEL):
None established
Component: Sodium Chloride
ACGIH Threshold Limit Value (TLV):
none established
OSHA Permissable Exposure Limit (PEL):
None established
8.2 Exposure Controls
Engineering Controls
A system of local and/or general exhaust is recommended to keep employee exposures below the Airborne Exposure Limits. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source.
Respiratory Protection
For conditions of use where exposure to the dust or mist is apparent, a full-face dust/mist respirator may be worn. For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-supplied respirator.
Eye Protection
Use chemical safety goggles and/or a full face shield where splashing is possible. Maintain eye wash fountain and quick-drench facilities in work area.
Skin Protection
Wear protective gloves and clean body covering clothing.
SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on Basic Physical & Chemical Properties
| a. Appearance | Clear, colorless solution | b. Odor | None |
| c. Odor Threshold | N.A. | d. pH | 7 |
| e. Melting/Freezing Point (oC) | 0 | f. Boiling point (oC) | 104 |
| g. Flash Point (oC) | N.A. | h. Evaporation Rate | N.A. |
| i. Flammability | N.A. | j. Upper/Lower Flammability or Explosive Limits | N.A. |
| k. Vapor Pressure | N.A. | l. Vapor Density (Air = 1) | N.A. |
| m. Relative Density | 1.05 | n. Water Solubility | Miscible |
| o. Partition Coefficient n-octanol/water | Mixture | p. Autoignition Temperature (oC) | N.A. |
| q. Decomposition Temperature (oC) | Not applicable. | r. Viscosity | No data available. |
| s. Explosive Properties | N.A. | t. Oxidizing Properties | N.A. |
SECTION 10 - STABILITY AND REACTIVITY
10.1 Reactivity
No Data
10.2 Chemical Stability
Stable under ordinary conditions of use and storage.
10.3 Possibility of Hazardous Reactions
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
10.4 Conditions to Avoid
No information found.
10.5 Incompatible Materials
Tris-Base:
No incompatibility data found.
Potassium Chloride:
Bromine trifluoride; potassium permanganate plus sulfuric acid.
Polysorbate 20:
Strong oxidizers, acids.
Water:
No incompatibility data found.
Sodium Chloride:
No incompatibility data found.
10.6 Hazardous Decomposition Products
Oxides of the contained metal and halogen, possibly also free, or ionic halogen
SECTION 11 - TOXICOLOGICAL INFORMATION
Product LD50 Values
Oral Rat LD50 (mg/kg)
260000
Dermal Rabbit LD50 (mg/kg)
Component Cancer List Status
| NTP Carcinogen | |||
| Known | Anticipated | IARC Category | |
|---|---|---|---|
| Tris-Base | No | No | None |
| Potassium Chloride | No | No | None |
| Polysorbate 20 | No | No | none |
| Water | No | No | None |
| Sodium Chloride | No | No | None |
Potential Health Effects
Inhalation
Tris-Base
Causes irritation to the respiratory tract.
Potassium Chloride
May cause respiratory tract irritation. Can produce delayed pulmonary edema.
Polysorbate 20
Not expected to be a health hazard by inhalation.
Water
No information found.
Sodium Chloride
May cause respiratory tract irritation.
Ingestion
Tris-Base
Causes irritation and reddening to the mucous membranes of the mouth, esophagus, and gastrointestinal tract.
Potassium Chloride
May produce weakness and circulatory problems. May affect heart. In severe cases, ingestion may be fatal.
Polysorbate 20
Large doses may produce abdominal spasms, diarrhea.
Water
No information found.
Sodium Chloride
Ingestion of large amounts may cause gastrointestinal irritation. Ingestion of large amounts may cause nausea and vomiting, rigidity or convulsions. Continued exposure can produce a coma, dehydration and internal organ congestion.
Skin
Tris-Base
Causes irritation to the skin.
Potassium Chloride
Contact may cause irritation or rash, particulary with moist skin.
Polysorbate 20
May cause irritation or sensitization in sensitive individuals.
Water
No information found.
Sodium Chloride
May cause skin irritation.
Eyes
Tris-Base
Causes irritation to the eyes.
Potassium Chloride
Potassium chloride is moderate eye irritant. Redness, tearing, possible abrasion can occur.
Polysorbate 20
May cause irritation.
Water
No information found.
Sodium Chloride
May cause eye irritation.
Carcinogenicity
Tris-Base
Not listed as a carcinogen by NTP or IARC.
Potassium Chloride
Not listed by ACGIH, IARC, NIOSH, NTP, OR OSHA.
Polysorbate 20
Not listed as a known or anticipated carcinogen by NTP or IARC.
Water
No information available.
Sodium Chloride
Not listed by ACGIH, IARC, NIOSH, NTP or OSHA.
Mutagenicity
Tris-Base
No information found.
Potassium Chloride
Unscheduled DNA Synthesis: Oral, rat = 1500 ug/kg.; Mutation in Microorganisms = Mouse, Lymphocyte = 2048 mg/L.; DNA Damage = Hamster, Ovary = 260 mmol/L.; Cytogenetic Analysis: Hamster, Lung = 12 gm/L
Polysorbate 20
No information found.
Water
No information available.
Sodium Chloride
See actual entry in RTECS for complete information.
Reproductive Toxicity
Tris-Base
No information found.
Potassium Chloride
No information available.
Polysorbate 20
No information found.
Water
No information available.
Sodium Chloride
No data available.
Teratogenic Effects
Tris-Base
No information found.
Potassium Chloride
No information available.
Polysorbate 20
No information found.
Water
No information available.
Sodium Chloride
No information found.
Routes of Entry
Tris-Base
Ingestion.
Potassium Chloride
Ingestion, inhalation, skin and eye contact.
Polysorbate 20
No information found.
Water
No information available
Sodium Chloride
No information found.
Target Organ Statement
Tris-Base
No information available.
Potassium Chloride
Polysorbate 20
No information found.
Water
No information available
Sodium Chloride
No information found.
SECTION 12 - ECOLOGICAL INFOMATION
12.1 Toxicity
COMPONENT: Tris-Base
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | LC50 460mg/l (Golden ide) | EC50: 59.8 mg/L (Daphnia) | EC50: 473mg/l @ 48 hrs | CE50>1000mg/L (3hrs) |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: Potassium Chloride
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | LC50 (96hr, Fathead minnow) 880mg/L | EC50 (48hr, Daphnia) 440-880 mg/L | EC50 (72hrs) >100mg/L | EC50 (3hr) >1000mg/L |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: Polysorbate 20
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: Water
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: Sodium Chloride
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | LC50 (96hrs, bluegill) 5480 mg/L | LC50 (48 hr, daphnia) 874mg/L | LC50 (120hrs) 2430 mg/L | No data |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | LD50 (house sparrow) 3500mg/kg | No data | IC50 (7 days) 500-1950mg/kg soil | No data |
12.2 Persistence and Degradability
Tris-Base
Readily Biodegradable (>97% degradation at 28 days)
Potassium Chloride
No data
Polysorbate 20
No data
Water
No data
Sodium Chloride
No data
12.3 Bioaccumulative Potential
Tris-Base
No data
Potassium Chloride
No data
Polysorbate 20
No data
Water
No data
Sodium Chloride
No data
12.4 Mobility in Soil
Tris-Base
Log Koc 1.57-1.85
Potassium Chloride
No data
Polysorbate 20
No data
Water
No data
Sodium Chloride
No data
12.5 Results of PBT and vPvB Assessment
Tris-Base
Not a PBT or vPvB
Potassium Chloride
No data
Polysorbate 20
No data
Water
No data
Sodium Chloride
Not PBT or vPvB
12.6 Other Adverse Effects
Tris-Base
None
Potassium Chloride
None
Polysorbate 20
None
Water
None
Sodium Chloride
None
SECTION 13 - DISPOSAL CONSIDERATIONS
13.1 Waste Treatment Methods
Offer surplus or non-recyclable product to licensed disposal company. Disposal is subject to user compliance with applicable law and product characteristics at time of disposal. Dispose of packaging as product.
SECTION 14 - TRANSPORT INFORMATION
| ADR/RID | IATA | IMO | DOT | |
|---|---|---|---|---|
| 14.1 UN Number | N.A. | N.A. | N.A. | N.A. |
| 14.2 Shipping Name | Not regulated. | Not regulated. | Not regulated. | Not regulated. |
| 14.3 Hazard Class | N.A. | N.A. | N.A. | N.A. |
| 14.4 Packing Group | N.A. | N.A. | N.A. | N.A. |
| 14.5 Environmental Hazards | N.A. | N.A. | Not regulated | N.A. |
| 14.6 Special Precautions | N.A. | N.A. | N.A. | N.A. |
SECTION 15 - REGULATORY INFORMATION
15.1 Safety, Health and Environmental Regulations/Legislation Specific for the Substance/Mixture
United States
TSCA Regulatory Statement
All intentional ingredients are listed on the TSCA Inventory.
SARA 311/312 Hazard Categories
| Component | Fire | Pressure | Reactivity | Acute | Chronic |
|---|---|---|---|---|---|
| Tris-Base | No | No | No | Yes | No |
| Potassium Chloride | No | No | No | Yes | No |
| Polysorbate 20 | No | No | No | Yes | No |
| Water | No | No | No | No | No |
| Sodium Chloride | No | No | No | Yes | No |
Europe
EEC Regulatory
All intentional ingredients are listed on the European EINECS Inventory.
SECTION 16 - OTHER INFORMATION
Revisional Updates
4/26/2019 - Updated Section 1.4
5/29/2015 - Updated Sections 2.1 and 3.2
6/27/2013- Released Version 1.0
NFPA Codes
Health 1 Flammability 0 Reactivity 0
Dangers
Tris-Base
H315 - Causes skin irritation.
H319 - Causes serious eye irritation.
H335 - May cause respiratory irritation.
Potassium Chloride
None
Polysorbate 20
None
Water
None
Sodium Chloride
None
MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without guarantee. Conditions of the use and suitability of the product for particular uses are beyond our control. All risks of use of the product are therefore assumed by the user. Nothing is intended as a recommendation for uses which infringe valid patents or as extending license under valid patents. Appropriate warnings and safe handling procedures should be provided to handlers and users.