Version 3.0
Date revised: 4/26/2019
SAFETY DATA SHEET
Conforms to regulation (EC) no. EU 453/2010
SECTION 1 - IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING
1.1 Product Identifier
1.2 Relevant Identified Uses of the Substance/Mixture and Uses Advised Against
Investigational research by professional users
1.3 Details of the Supplier of the Safety Data Sheet
| Manufacturer National Diagnostics 305 Patton Drive Atlanta, GA 30036 (404) 699-2121 (800) 526-3867 info@nationaldiagnostics.com |
1.4 Emergency Telephone Number
ChemTel Inc.
Contract number MIS8894340
1-800 255-3924 (United States, Canada, Puerto Rico & US Virgin Islands)
01-800-099-0731 (Mexico)
400-120-0751 (China)
000-800-100-4086 (India)
1-300-954-583 (Australia)
0-800-591-6042 (Brazil)
+1-813-255-3924 (All other regions)
SECTION 2 - HAZARDS IDENTIFICATION
2.1 Classification of the Substance or Mixture
Classification according to Regulation (EC) No. 1272/2008 [EU-GHS/CLP]
H302 - Acute Toxicity-Oral (Category 4)
H312 - Acute Toxicity-Dermal (Category 4)
H315 - Skin Corrosion/Irritation (Category 2)
H317 - Skin Sensitizer (Category 1)
H319 - Serious Eye Damage/Eye Irritation (Category 2A)
H332 - Acute Toxicity-Inhalation (Category 4)
H340 - Germ Cell Mutagenicity (Category 1B)
H350 - Carcinogenicity (Category 1B)
H361 - Toxic to Reproduction (Category 2)
H372 - Specific Target Organ Toxicity Following Repeated Exposure (Category 1)
2.2 Label Elements
| GHS Label Elements | ||
 |  | DANGER H302 - Harmful if swallowed |
2.3 Other Hazards
None found.
SECTION 3 - COMPOSITION/INFORMATION ON INGREDIENTS
3.2 Mixture
Chemical Names/Description
Aqueous solution of acrylamides and buffer salts.
Component List
| Component | % Comp. | CAS # | EC # | 1278/2008 Classification |
|---|---|---|---|---|
| ACRYLAMIDES | 12% | 79-06-1 | 201-173-7 | H301, H312, H315, H317, H319, H332, H340, H350, H361, H372 |
| Proprietary Electrophoresis Buffers | <20% | N.A. | ||
| Tris-Base | <5% | 77-86-1 | 201-064-4 | H315, H319, H335 |
| Glycine | <5% | 56-40-6 | 200-272-2 | N.A. |
SECTION 4 - FIRST AID MEASURES
4.1 Description of First Aid Measures
Inhalation
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician.
Ingestion
Induce vomiting immediately as directed by medical personnel. Never give anything by mouth to an unconscious person. Call a physician.
Skin
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.
Eyes
Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally. Get medical attention immediately.
4.2 Most Important Symptoms and Effects, Both Acute and Delayed
Inhalation
ACRYLAMIDES:
Contact with this material by inhalation of mist may cause nervous system effects. See ingestion effects for more details.
Proprietary Electrophoresis Buffers:
Tris-Base:
Coughing, shortness of breath.
Glycine:
Coughing, soreness in the respiratory tract, chest tightening, difficulty breathing.
Ingestion
ACRYLAMIDES:
Contact with this material by any route (eyes/skin, inhalation or ingestion) may cause nervous system effects (neurotoxicity). These effects can result from a single overexposure but are more likely to occur after repeated exposures to small amounts over a period of several days or weeks. Signs and symptoms of toxic effects include increased sweating of the hands and feet, numbness, tingling and weakness in the extremeties, unsteady gait and decreased reflexes
Proprietary Electrophoresis Buffers:
Tris-Base:
Symptoms may include nausea, vomiting, and diarrhea. Large oral doses may cause weakness, collapse, blood clotting, and coma. The estimated lethal dose of Tris Base is 50 grams dry solid.
Glycine:
Nausea.
Skin
ACRYLAMIDES:
Acrylamide is readily absorbed through unbroken skin. If the exposure route is dermal, the signs and symptoms described above under 'Signs and Symptoms of Overexposure - Ingestion' may be preceded by peeling and redness of skin at the areas of exposure, normally the hands and feet.
Proprietary Electrophoresis Buffers:
Tris-Base:
Redness, itching, and pain.
Glycine:
Mild reddening.
Eyes
ACRYLAMIDES:
Contact with this material by eyes may cause nervous system effects. See 'Signs and Symptoms of Overexposure - Ingestion' above for more details.
Proprietary Electrophoresis Buffers:
Tris-Base:
Redness, itching, and pain.
Glycine:
Mild reddening.
4.3 Indication of Any Immediate Medical Attention and Special Treatment Needed
Unknown/not applicable
SECTION 5 - FIRE FIGHTING MEASURES
5.1 Extinguishing media
Use media appropriate to the primary cause of fire.
5.2 Special Hazards Arising from the Substance/Mixture
Hazardous Combustion Products
Thermal decomposition products may include toxic oxides of nitrogen and carbon.
Hazardous Decomposition Products
Upon heating, may produce ammonia, nitrogen oxides, cyanuric acid, cyanic acid, biuret, carbon dioxide, carbon monoxide, and hydrogen.
Hazardous Polymerization
May occur
5.3 Advice for Firefighters
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.
5.4 Further Information
No data available.
SECTION 6 - ACCIDENTAL RELEASE MEASURES
6.1 Personal Precautions
If water solvent has evaporated, wear NIOSH approved air-purifying respirator.
6.2 Environmental Precautions
Prevent discharge into the environment. Dike spills and stop leakage where practical. Do not allow material to enter drains.
6.3 Methods and Materials for Containment and Cleaning Up
Contain and clean up spill immediately, prevent from entering floor drains. Contain liquids using absorbents. Shovel all spill materials into disposal drum. Scrub spill area with detergent, flush with copious amounts of water.
6.4 References to Other Sections
For disposal information see Section 13. For protective clothing and equipment see Section 8.
SECTION 7 - HANDLING AND STORAGE
7.1 Precautions for Safe Handling
Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling. Wear special protective equipment (Sec. 8) where exposures may exceed established levels.
7.2 Conditions for Safe Storage (including any incompatibles)
Keep in a tightly closed container, stored in a cooled, dry, ventilated area. Protect from physical damage. Isolate from incompatible materials (section 10).
Incompatibles
ACRYLAMIDES:
Acrylamide reacts with acids, oxidizing agents, and bases. Spontaneously reacts with hydroxyl-, amino-, and sulfhydryl- containing compounds. Avoid vinyl polymerization initiators or contamination with aluminum, iron, copper, brass, and bronze.
Proprietary Electrophoresis Buffers:
Tris-Base:
No incompatibility data found.
Glycine:
No incompatibility data found.
7.3 Specific End Uses
Investigational research by professional users
SECTION 8 - EXPOSURE CONTROLS/PERSONAL PRECAUTIONS
8.1 Control Parameters
Component: ACRYLAMIDES
ACGIH Threshold Limit Value (TLV):
0.03 mg/m3 (TWA) (skin) for solid
OSHA Permissable Exposure Limit (PEL):
0.3 mg/m3 (TWA) (skin) for solid
Component: Proprietary Electrophoresis Buffers
ACGIH Threshold Limit Value (TLV):
None established
OSHA Permissable Exposure Limit (PEL):
None established
Component: Tris-Base
ACGIH Threshold Limit Value (TLV):
none established
OSHA Permissable Exposure Limit (PEL):
none established
Component: Glycine
ACGIH Threshold Limit Value (TLV):
15 mg/m3
OSHA Permissable Exposure Limit (PEL):
None established
8.2 Exposure Controls
Engineering Controls
A system of local and/or general exhaust is recommended to keep employee exposures below the Airborne Exposure Limits. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source.
Respiratory Protection
If exposure limits are exceeded, wear a full-face respirator with organic vapor cartridge and high efficiency dust mist filter. Beyond fifty times exposure limits or when exposure levels are not known, wear a full-face piece positive pressure respirator.
Eye Protection
Use chemical safety goggles and/or a full face shield where splashing is possible. Maintain eye wash fountain and quick-drench facilities in work area.
Skin Protection
Wear impervious protective clothing, including boots, gloves, lab coat, apron or coveralls, as appropriate, to prevent skin contact.
SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on Basic Physical & Chemical Properties
| a. Appearance | Clear, colorless solution | b. Odor | None |
| c. Odor Threshold | N.A. | d. pH | Neutral |
| e. Melting/Freezing Point (oC) | 0 | f. Boiling point (oC) | 104.4 |
| g. Flash Point (oC) | N.A. | h. Evaporation Rate | 1.0 |
| i. Flammability | N.A. | j. Upper/Lower Flammability or Explosive Limits | N.A. |
| k. Vapor Pressure | Water | l. Vapor Density (Air = 1) | N.A. |
| m. Relative Density | 1.15 | n. Water Solubility | Soluble |
| o. Partition Coefficient n-octanol/water | Mixture | p. Autoignition Temperature (oC) | N.A. |
| q. Decomposition Temperature (oC) | Not applicable. | r. Viscosity | No data available. |
| s. Explosive Properties | N.A. | t. Oxidizing Properties | N.A. |
SECTION 10 - STABILITY AND REACTIVITY
10.1 Reactivity
Reacts with vinyl polymerization initiators to produce an exothermic polymerization..
10.2 Chemical Stability
Stable under ordinary conditions of use and storage.
10.3 Possibility of Hazardous Reactions
May occur
10.4 Conditions to Avoid
Heat, shock, UV light, and incompatibles.
10.5 Incompatible Materials
ACRYLAMIDES:
Acrylamide reacts with acids, oxidizing agents, and bases. Spontaneously reacts with hydroxyl-, amino-, and sulfhydryl- containing compounds. Avoid vinyl polymerization initiators or contamination with aluminum, iron, copper, brass, and bronze.
Proprietary Electrophoresis Buffers:
Tris-Base:
No incompatibility data found.
Glycine:
No incompatibility data found.
10.6 Hazardous Decomposition Products
Upon heating, may produce ammonia, nitrogen oxides, cyanuric acid, cyanic acid, biuret, carbon dioxide, carbon monoxide, and hydrogen.
SECTION 11 - TOXICOLOGICAL INFORMATION
Product LD50 Values
Oral Rat LD50 (mg/kg)
1667
Dermal Rabbit LD50 (mg/kg)
7283
Component Cancer List Status
| NTP Carcinogen | |||
| Known | Anticipated | IARC Category | |
|---|---|---|---|
| ACRYLAMIDES | No | Yes | 2A |
| Proprietary Electrophoresis Buffers | No | No | None |
| Tris-Base | No | No | None |
| Glycine | No | No | None |
Potential Health Effects
Inhalation
ACRYLAMIDES
Inhalation of mist causes irritation to the respiratory tract. Symptoms may parallel ingestion.
Proprietary Electrophoresis Buffers
Tris-Base
Causes irritation to the respiratory tract.
Glycine
High concentrations of dust may be slightly irritating to the lungs.
Ingestion
ACRYLAMIDES
Toxic! May cause systemic poisoning. May cause drowsiness, tingling sensations, fatigue, weakness, stumbling, slurred speech, and shaking. May cause central and peripheral nervous system damage. Severe intoxication may cause permanent nerve damage. May affect reproductive system and act as a teratogen.
Proprietary Electrophoresis Buffers
Tris-Base
Causes irritation and reddening to the mucous membranes of the mouth, esophagus, and gastrointestinal tract.
Glycine
Large oral doses may cause nausea.
Skin
ACRYLAMIDES
May cause irritation and redness. Can be absorbed through the skin causing systemic poisoning; symptoms may parallel ingestion.
Proprietary Electrophoresis Buffers
Tris-Base
Causes irritation to the skin.
Glycine
May cause mild irritation to the skin.
Eyes
ACRYLAMIDES
Acrylamide solutions may cause eye irritation.
Proprietary Electrophoresis Buffers
Tris-Base
Causes irritation to the eyes.
Glycine
May cause mild irritation to the eyes.
Carcinogenicity
ACRYLAMIDES
Acrylamide is suspected as a cancer hazard. May cause cancer. Listed by NTP as a suspected carcinogen. Acrylamide is known to the State of California to cause cancer.
Proprietary Electrophoresis Buffers
Tris-Base
Not listed as a carcinogen by NTP or IARC.
Glycine
According to definitions of the U.S. Hazard Communication Standard and the Canadian WHMIS Regulation, this material is not listed on the NTP, IARC, ACGIH, or OSHA carcinogen lists, and there are no studies implicating components as cancer causing agents in humans or animals.
Mutagenicity
ACRYLAMIDES
Acrylamide was negative in the Ames assay both with and without metabolic activation.
Proprietary Electrophoresis Buffers
Tris-Base
No information found.
Glycine
No information found.
Reproductive Toxicity
ACRYLAMIDES
Acrylamide induced male reproductive toxicity has been demonstrated in Long-Evans rats where given greater than or equal to 15 mg/kg/day acrylamide orally by gavage for five consecutive days. In this study, males receiving greater than or equal to 15 mg/kg/day acrylamide had a reduced fertility index.
Proprietary Electrophoresis Buffers
Tris-Base
No information found.
Glycine
No information found.
Teratogenic Effects
ACRYLAMIDES
Not Available.
Proprietary Electrophoresis Buffers
Tris-Base
No information found.
Glycine
No information found.
Routes of Entry
ACRYLAMIDES
Contact with this material by any route of exposure (eye/skin, inhalation or ingestion) may cause serious adverse health consequences.
Proprietary Electrophoresis Buffers
Tris-Base
Ingestion.
Glycine
Inhalation of dust.
Target Organ Statement
ACRYLAMIDES
Not Available.
Proprietary Electrophoresis Buffers
Tris-Base
No information available.
Glycine
Persons with pre-existing skin or lung disorders may be more susceptible to irritation effects.
SECTION 12 - ECOLOGICAL INFOMATION
12.1 Toxicity
COMPONENT: ACRYLAMIDES
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | 96 hr LC50: 180ppm (Rainbow Trout) | 48-hour EC50: 98 mg/l (Daphnea) | ICA50 (growth inhibition): 67.7 mg/l (Selenastrum capricornutum) | No data |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: Proprietary Electrophoresis Buffers
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: Tris-Base
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | LC50 460mg/l (Golden ide) | EC50: 59.8 mg/L (Daphnia) | EC50: 473mg/l @ 48 hrs | CE50>1000mg/L (3hrs) |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: Glycine
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
12.2 Persistence and Degradability
ACRYLAMIDES
Readily biodegradable: The test material was found to degrade approximately 100% in 28 days in the OECD Closed Bottle Test (301D).
Proprietary Electrophoresis Buffers
No data
Tris-Base
Readily Biodegradable (>97% degradation at 28 days)
Glycine
No data
12.3 Bioaccumulative Potential
ACRYLAMIDES
No data
Proprietary Electrophoresis Buffers
No data
Tris-Base
No data
Glycine
No data
12.4 Mobility in Soil
ACRYLAMIDES
No data
Proprietary Electrophoresis Buffers
No data
Tris-Base
Log Koc 1.57-1.85
Glycine
No data
12.5 Results of PBT and vPvB Assessment
ACRYLAMIDES
not PBT or vPvB
Proprietary Electrophoresis Buffers
No data
Tris-Base
Not a PBT or vPvB
Glycine
No data
12.6 Other Adverse Effects
ACRYLAMIDES
None
Proprietary Electrophoresis Buffers
None
Tris-Base
None
Glycine
None
SECTION 13 - DISPOSAL CONSIDERATIONS
13.1 Waste Treatment Methods
Offer surplus or non-recyclable product to licensed disposal company. Disposal is subject to user compliance with applicable law and product characteristics at time of disposal. Dispose of packaging as product.
SECTION 14 - TRANSPORT INFORMATION
| ADR/RID | IATA | IMO | DOT | |
|---|---|---|---|---|
| 14.1 UN Number | N.A. | N.A. | N.A. | N.A. |
| 14.2 Shipping Name | Not Regulated | Not Regulated | Not Regulated | Not Regulated |
| 14.3 Hazard Class | N.A. | N.A. | N.A. | N.A. |
| 14.4 Packing Group | N.A. | N.A. | N.A. | N.A. |
| 14.5 Environmental Hazards | N.A. | N.A. | Not regulated | N.A. |
| 14.6 Special Precautions | N.A. | N.A. | N.A. | N.A. |
SECTION 15 - REGULATORY INFORMATION
15.1 Safety, Health and Environmental Regulations/Legislation Specific for the Substance/Mixture
United States
TSCA Regulatory Statement
All intentional ingredients are listed on the TSCA Inventory.
SARA 311/312 Hazard Categories
| Component | Fire | Pressure | Reactivity | Acute | Chronic |
|---|---|---|---|---|---|
| ACRYLAMIDES | No | No | No | Yes | Yes |
| Proprietary Electrophoresis Buffers | No | No | No | Yes | Yes |
| Tris-Base | No | No | No | Yes | No |
| Glycine | No | No | No | No | No |
Europe
EEC Regulatory
All intentional ingredients are listed on the European EINECS Inventory.
SECTION 16 - OTHER INFORMATION
Revisional Updates
4/26/2019 - Updated Section 1.4
5/29/2015 - Updated Sections 2.1 and 3.2
1/28/2015 - Updated Sections 2.1 and 2.2
7/16/2013 - Released Version 1.0
NFPA Codes
Health 2 Flammability 1 Reactivity 1
Dangers
ACRYLAMIDES
H302 - Harmful if swallowed.
H312 - Harmful in contact with skin.
H315 - Causes skin irritation.
H317 - May cause an allergic skin reaction.
H319 - Causes serious eye irritation.
H332 - Harmful if inhaled.
H340 - May cause genetic defects.
H350 - May cause cancer.
H361 - Suspected of damaging fertility or the unborn child.
H372 - Causes damage to organs through prolonged or repeated exposure.
Proprietary Electrophoresis Buffers
None
Tris-Base
H315 - Causes skin irritation.
H319 - Causes serious eye irritation.
H335 - May cause respiratory irritation.
Glycine
None
MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without guarantee. Conditions of the use and suitability of the product for particular uses are beyond our control. All risks of use of the product are therefore assumed by the user. Nothing is intended as a recommendation for uses which infringe valid patents or as extending license under valid patents. Appropriate warnings and safe handling procedures should be provided to handlers and users.