Version 3.0
Date revised: 4/26/2019
SAFETY DATA SHEET
Conforms to regulation (EC) no. EU 453/2010
SECTION 1 - IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING
1.1 Product Identifier
1.2 Relevant Identified Uses of the Substance/Mixture and Uses Advised Against
Investigational research by professional users
1.3 Details of the Supplier of the Safety Data Sheet
| Manufacturer National Diagnostics 305 Patton Drive Atlanta, GA 30036 (404) 699-2121 (800) 526-3867 info@nationaldiagnostics.com |
1.4 Emergency Telephone Number
ChemTel Inc.
Contract number MIS8894340
1-800 255-3924 (United States, Canada, Puerto Rico & US Virgin Islands)
01-800-099-0731 (Mexico)
400-120-0751 (China)
000-800-100-4086 (India)
1-300-954-583 (Australia)
0-800-591-6042 (Brazil)
+1-813-255-3924 (All other regions)
SECTION 2 - HAZARDS IDENTIFICATION
2.1 Classification of the Substance or Mixture
Classification according to Regulation (EC) No. 1272/2008 [EU-GHS/CLP]
H302 - Acute Toxicity-Oral (Category 4)
H312 - Acute Toxicity-Dermal (Category 4)
H315 - Skin Corrosion/Irritation (Category 2)
H319 - Serious Eye Damage/Eye Irritation (Category 2A)
H332 - Acute Toxicity-Inhalation (Category 4)
2.2 Label Elements
| GHS Label Elements | ||
 | WARNING H302 - Harmful if swallowed | |
2.3 Other Hazards
None found.
SECTION 3 - COMPOSITION/INFORMATION ON INGREDIENTS
3.2 Mixture
Chemical Names/Description
Aqueous solution of tris base, SDS, glyerol, mercaptoethanol (and proteins <1%)
Component List
| Component | % Comp. | CAS # | EC # | 1278/2008 Classification |
|---|---|---|---|---|
| 2-Mercaptoethanol | 2 | 60-24-2 | 200-464-6 | H301, H310, H315, H317, H318, H331, H373, H400, H410 |
| Tris-Base | 1.5 | 77-86-1 | 201-064-4 | H315, H319, H335 |
| SDS | 4.4 | 151-21-3 | 205-788-1 | H302, H315, H319, H335 |
| Glycerol | 20 | 56-81-5 | 200-289-5 | N.A. |
SECTION 4 - FIRST AID MEASURES
4.1 Description of First Aid Measures
Inhalation
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician.
Ingestion
Do not induce vomiting. If swallowed and the person is conscious, immediately give large amounts of water. Get medical attention.
Skin
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.
Eyes
Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally. Get medical attention immediately.
4.2 Most Important Symptoms and Effects, Both Acute and Delayed
Inhalation
2-Mercaptoethanol:
Symptoms may include coughing, sore throat, shortness of breath, headaches, nausea, and vomiting. Prolonged exposure can cause CNS stimulation.
Tris-Base:
Coughing, shortness of breath.
SDS:
Coughing, shortness of breath. May cause allergic reaction in sensitive individuals.
Glycerol:
Inhalation symptoms unlikely.
Ingestion
2-Mercaptoethanol:
Symptoms may include sore throat, abdominal pain, and vomiting.
Tris-Base:
Symptoms may include nausea, vomiting, and diarrhea. Large oral doses may cause weakness, collapse, blood clotting, and coma. The estimated lethal dose of Tris Base is 50 grams dry solid.
SDS:
Nausea and diarrhea.
Glycerol:
Nausea, headache, diarrhea.
Skin
2-Mercaptoethanol:
Symptoms may include skin irritation.
Tris-Base:
Redness, itching, and pain.
SDS:
Causes dryness and a rash on continued exposure.
Glycerol:
Irritation.
Eyes
2-Mercaptoethanol:
Symptoms may include redness and pain.
Tris-Base:
Redness, itching, and pain.
SDS:
Causes redness and pain.
Glycerol:
Irritation.
4.3 Indication of Any Immediate Medical Attention and Special Treatment Needed
Unknown/not applicable
SECTION 5 - FIRE FIGHTING MEASURES
5.1 Extinguishing media
Use media appropriate to the primary cause of fire.
5.2 Special Hazards Arising from the Substance/Mixture
Hazardous Combustion Products
Thermal decomposition products may include toxic oxides of nitrogen, sulfur, and carbon.
Hazardous Decomposition Products
Toxic gases may be involved in a fire. Glycerin decomposes upon headting above 290C, forming corrosive gas (acrolein).
Hazardous Polymerization
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
5.3 Advice for Firefighters
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.
5.4 Further Information
No data available.
SECTION 6 - ACCIDENTAL RELEASE MEASURES
6.1 Personal Precautions
Wear appropriate protective equipment as specified in Section 8.
6.2 Environmental Precautions
Prevent discharge into the environment. Dike spills and stop leakage where practical. Do not allow material to enter drains.
6.3 Methods and Materials for Containment and Cleaning Up
Contain and clean up spill immediately, prevent from entering floor drains. Contain liquids using absorbents. Shovel all spill materials into disposal drum. Scrub spill area with detergent, flush with copious amounts of water.
6.4 References to Other Sections
For disposal information see Section 13. For protective clothing and equipment see Section 8.
SECTION 7 - HANDLING AND STORAGE
7.1 Precautions for Safe Handling
Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
7.2 Conditions for Safe Storage (including any incompatibles)
Keep frozen until ready to use. Protect from physical damage. Isolate from incompatible materials (section 10).
Incompatibles
2-Mercaptoethanol:
Oxidizing agents, moisture, Avoid contact with metals.
Tris-Base:
No incompatibility data found.
SDS:
Strong oxidizers, acids.
Glycerol:
Strong oxidizers. Can react violently with acetic anhydride, calcium oxychloride, chromium oxides and alkali metal hydrides.
7.3 Specific End Uses
Investigational research by professional users
SECTION 8 - EXPOSURE CONTROLS/PERSONAL PRECAUTIONS
8.1 Control Parameters
Component: 2-Mercaptoethanol
ACGIH Threshold Limit Value (TLV):
Not Established
OSHA Permissable Exposure Limit (PEL):
AIHA WEEL 0.2ppm, 8 hr. TWA
Component: Tris-Base
ACGIH Threshold Limit Value (TLV):
none established
OSHA Permissable Exposure Limit (PEL):
none established
Component: SDS
ACGIH Threshold Limit Value (TLV):
None established
OSHA Permissable Exposure Limit (PEL):
None established
Component: Glycerol
ACGIH Threshold Limit Value (TLV):
10 mg/m3
OSHA Permissable Exposure Limit (PEL):
None established
8.2 Exposure Controls
Engineering Controls
A system of local and/or general exhaust is recommended to keep employee exposures below the Airborne Exposure Limits. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source.
Respiratory Protection
If the exposure limit is exceeded, wear a supplied air, full-facepiece respirator, airlined hood, or full-facepiece self-contained breathing apparatus.
Eye Protection
Use chemical safety goggles and/or a full face shield where splashing is possible. Maintain eye wash fountain and quick-drench facilities in work area.
Skin Protection
Wear impervious protective clothing, including boots, gloves, lab coat, apron or coveralls, as appropriate, to prevent skin contact.
SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on Basic Physical & Chemical Properties
| a. Appearance | Clear, colorless solution | b. Odor | None |
| c. Odor Threshold | N.A. | d. pH | 6.8 |
| e. Melting/Freezing Point (oC) | -5 | f. Boiling point (oC) | 105 |
| g. Flash Point (oC) | N.A. | h. Evaporation Rate | Water |
| i. Flammability | N.A. | j. Upper/Lower Flammability or Explosive Limits | N.A. |
| k. Vapor Pressure | Water | l. Vapor Density (Air = 1) | Water |
| m. Relative Density | No information. | n. Water Solubility | Soluble |
| o. Partition Coefficient n-octanol/water | mixture | p. Autoignition Temperature (oC) | N.A. |
| q. Decomposition Temperature (oC) | Not applicable. | r. Viscosity | No data available. |
| s. Explosive Properties | N.A. | t. Oxidizing Properties | not an oxidizer |
SECTION 10 - STABILITY AND REACTIVITY
10.1 Reactivity
Non reactive under normal conditions of use and storage.
10.2 Chemical Stability
Stable under recommended conditions of use and storage.
10.3 Possibility of Hazardous Reactions
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
10.4 Conditions to Avoid
Heat, incompatibles.
10.5 Incompatible Materials
2-Mercaptoethanol:
Oxidizing agents, moisture, Avoid contact with metals.
Tris-Base:
No incompatibility data found.
SDS:
Strong oxidizers, acids.
Glycerol:
Strong oxidizers. Can react violently with acetic anhydride, calcium oxychloride, chromium oxides and alkali metal hydrides.
10.6 Hazardous Decomposition Products
Toxic gases may be involved in a fire. Glycerin decomposes upon headting above 290C, forming corrosive gas (acrolein).
SECTION 11 - TOXICOLOGICAL INFORMATION
Product LD50 Values
Oral Rat LD50 (mg/kg)
12200
Dermal Rabbit LD50 (mg/kg)
7500
Component Cancer List Status
| NTP Carcinogen | |||
| Known | Anticipated | IARC Category | |
|---|---|---|---|
| 2-Mercaptoethanol | No | No | None |
| Tris-Base | No | No | None |
| SDS | No | No | None |
| Glycerol | No | No | None |
Potential Health Effects
Inhalation
2-Mercaptoethanol
Vapors irritate the mucous membranes and respiratory tract.
Tris-Base
Causes irritation to the respiratory tract.
SDS
Dust causes irritation to the respiratory tract.
Glycerol
Due to the low vapor pressure, inhalation of the vapors at room temperatures in unlikely. Inhalation of mist may cause irritation of respiratory tract.
Ingestion
2-Mercaptoethanol
Toxic. Harmful if swallowed. Sore throat, abdominal pain and vomiting may occur.
Tris-Base
Causes irritation and reddening to the mucous membranes of the mouth, esophagus, and gastrointestinal tract.
SDS
Large doses may cause gastrointestinal distress.
Glycerol
Low toxicity. May cause nausea, headache, diarrhea.
Skin
2-Mercaptoethanol
Toxic. Causes skin irritation and may be absorbed in the body in toxic quantities.
Tris-Base
Causes irritation to the skin.
SDS
Mildly irritating to skin. May cause allergic skin reactions.
Glycerol
May cause irritation.
Eyes
2-Mercaptoethanol
Vapors irritate the eyes with redness and pain. Splashes may cause severe irritation.
Tris-Base
Causes irritation to the eyes.
SDS
Causes irritation to the eyes.
Glycerol
May cause irritation.
Carcinogenicity
2-Mercaptoethanol
Substance is neither a known nor an anticipated carcinogen. Not listed by NTP, IARC, or OSHA.
Tris-Base
Not listed as a carcinogen by NTP or IARC.
SDS
Not listed as a known or suspected carcinogen by NTP or IARC.
Glycerol
According to definitions of the U.S. Hazard Communication Standard and the Canadian WHMIS Regulation, this material is not listed on the NTP, IARC, ACGIH, or OSHA carcinogen lists, and there are no studies implicating components as cancer causing agents in humans or animals.
Mutagenicity
2-Mercaptoethanol
No information available.
Tris-Base
No information found.
SDS
Has caused mutagenic effects on laboratory animals.
Glycerol
No information found.
Reproductive Toxicity
2-Mercaptoethanol
No information available.
Tris-Base
No information found.
SDS
Has caused mutagenic effects on laboratory animals.
Glycerol
No information found.
Teratogenic Effects
2-Mercaptoethanol
No information available.
Tris-Base
No information found.
SDS
No information found.
Glycerol
No information found.
Routes of Entry
2-Mercaptoethanol
Toxic effects possible by inhalation, ingestion, and skin absorption.
Tris-Base
Ingestion.
SDS
No information found.
Glycerol
No information found.
Target Organ Statement
2-Mercaptoethanol
Behavioral: Tremor, convulsion, excitement, muscle contraction/spasticity. Lungs, thorax: Respiratory depression. GI: Changes in structure/function of salivary glands.
Tris-Base
No information available.
SDS
Persons with pre-existing skin disorders or impaired respiratory function may be more susceptible to the effects of the substance.
Glycerol
Persons with pre-existing skin disorders or eye problems or impaired liver or kidney function may be more susceptible to the effects of this substance.
SECTION 12 - ECOLOGICAL INFOMATION
12.1 Toxicity
COMPONENT: 2-Mercaptoethanol
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | LC50 (96hr, golden orfe) 37 mg/L | EC50 (daphnia, 48 hr) 0.4 mg/L | LC50 (96h) : 19 mg/L | EC50 (17 h) : 113 mg/l |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: Tris-Base
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | LC50 460mg/l (Golden ide) | EC50: 59.8 mg/L (Daphnia) | EC50: 473mg/l @ 48 hrs | CE50>1000mg/L (3hrs) |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: SDS
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | The 96 hr LC50 of dodecyl sulfate to Fathead minnows was 29 mg/L | LC50 (Ceriodaphnia dubia, 48-hr): 5.55 mg/L | EC50>120mg/L | IC50 (3 hrs): 480 mg/L |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | EC50 (72hr, Cicer arietinum ) 361 mg/L | No data |
COMPONENT: Glycerol
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | No data | No data | EC3 : >10,000 mg/L | EC50 (16hrs) > 10000 mg/L |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
12.2 Persistence and Degradability
2-Mercaptoethanol
Biodegradable (90% in 28 days)
Tris-Base
Readily Biodegradable (>97% degradation at 28 days)
SDS
Readily biodegradable (>95% degradation in 28 days)
Glycerol
Readily biodegradable
12.3 Bioaccumulative Potential
2-Mercaptoethanol
No data
Tris-Base
No data
SDS
No data
Glycerol
No data
12.4 Mobility in Soil
2-Mercaptoethanol
Koc 1.325
Tris-Base
Log Koc 1.57-1.85
SDS
Log Koc 1.545
Glycerol
No data
12.5 Results of PBT and vPvB Assessment
2-Mercaptoethanol
Not a PBT or vPvB
Tris-Base
Not a PBT or vPvB
SDS
Not PBT vPvB
Glycerol
Not PBT or vPvB
12.6 Other Adverse Effects
2-Mercaptoethanol
None
Tris-Base
None
SDS
None
Glycerol
None
SECTION 13 - DISPOSAL CONSIDERATIONS
13.1 Waste Treatment Methods
Offer surplus or non-recyclable product to licensed disposal company. Disposal is subject to user compliance with applicable law and product characteristics at time of disposal. Dispose of packaging as product.
SECTION 14 - TRANSPORT INFORMATION
| ADR/RID | IATA | IMO | DOT | |
|---|---|---|---|---|
| 14.1 UN Number | N.A. | N.A. | N.A. | N.A. |
| 14.2 Shipping Name | Not regulated. | Not regulated. | Not regulated. | Not regulated. |
| 14.3 Hazard Class | N.A. | N.A. | N.A. | N.A. |
| 14.4 Packing Group | N.A. | N.A. | N.A. | N.A. |
| 14.5 Environmental Hazards | N.A. | N.A. | Not regulated | N.A. |
| 14.6 Special Precautions | N.A. | N.A. | N.A. | N.A. |
SECTION 15 - REGULATORY INFORMATION
15.1 Safety, Health and Environmental Regulations/Legislation Specific for the Substance/Mixture
United States
TSCA Regulatory Statement
All intentional ingredients are listed on the TSCA Inventory.
SARA 311/312 Hazard Categories
| Component | Fire | Pressure | Reactivity | Acute | Chronic |
|---|---|---|---|---|---|
| 2-Mercaptoethanol | Yes | No | No | Yes | No |
| Tris-Base | No | No | No | Yes | No |
| SDS | No | No | No | Yes | Yes |
| Glycerol | No | No | No | Yes | Yes |
Europe
EEC Regulatory
All intentional ingredients are listed on the European EINECS Inventory.
SECTION 16 - OTHER INFORMATION
Revisional Updates
4/26/2019 - Updated Section 1.4
5/29/2015 - Updated Sections 2.1 and 3.2
10/11/2013 - Released Version 1.0
NFPA Codes
Health N.D. Flammability N.D. Reactivity N.D.
Dangers
2-Mercaptoethanol
H301 - Toxic if swallowed
H310 - Fatal in contact with skin.
H315 - Causes skin irritation.
H317 - May cause an allergic skin reaction.
H318 - Causes serious eye damage.
H331 - Toxic if inhaled.
H373 - May cause damage to organs through prolonged or repeated exposure.
H401 - Toxic to aquatic life.
H410 - Very toxic to aquatic life with long lasting effects.
Tris-Base
H315 - Causes skin irritation.
H319 - Causes serious eye irritation.
H335 - May cause respiratory irritation.
SDS
H302 - Harmful if swallowed
H315 - Causes skin irritation.
H319 - Causes serious eye irritation.
H335 - May cause respiratory irritation.
Glycerol
None
MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without guarantee. Conditions of the use and suitability of the product for particular uses are beyond our control. All risks of use of the product are therefore assumed by the user. Nothing is intended as a recommendation for uses which infringe valid patents or as extending license under valid patents. Appropriate warnings and safe handling procedures should be provided to handlers and users.