In the absence of denaturants double stranded DNA retains its double helical structure, which gives it a rodlike form as it migrates through a gel
Sample preparation for native PAGE is straightforward.
Native PAGE gels are prepared by mixing an acrylamide/bisacrylamide monomer concentrate (AccuGel
The polymerase chain reaction (PCR) is a powerful technique which uses repetitive cycles of primer annealing, primer extension, and product denatu
PCR reactions produce product in a nonlinear pattern (See figure below).
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