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Overview of the Paraffin Technique

Once fixed, the tissue must be treated to allow the cutting of the thin sections required for viewing under the microscope.


Dehydration is usually carried out by transferring the tissue through solutions of increasing alcohol concentration, until 100% alcohol is reached

Clearing Tissue Sections

The step following dehydration is called "clearing" and consists of replacing the dehydrant with a substance that will be miscible with the embeddi

Mounting Tissue Sections

To preserve and support a stained section for light microscopy, it is mounted on a clear glass slide, and covered with a thin glass coverslip.


Immunohistochemistry is the application of antibody/antigen interactions to provide information about biological systems.


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