An aspect of LSC which must be considered in experimental design, is waste disposal.
Liquid scintillation counting of discrete samples is conceptually straightforward.
In a typical TLC experiment, the radioactivity is detected at two points: after TLC it is analyzed by autoradiography
A common radiotracer technique is to precipitate macromolecules (protein & DNA) with TCA or some other strong denaturant, collect the precipit
Samples of animal or plant tissue are rarely thin or small enough to allow for full counting efficiency.
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