SequaGel UreaGel 29:1 Concentrate

Protocol

Procedure

Mix UreaGel System Components

Determine how much UreaGel 29:1 Concentrate, Diluent, and Buffer you need to make your gels using the formulas below. Combine the necessary components in an Erlenmeyer flask. Swirl gently to mix.

For 100 ml Gel Casting Solution:

V_c = (V_t) (X)/25
V_b = 0.1 (V_t)
V_d = V_t – (V_c + V_b)

where:

V_c = UreaGel 29:1 Concentrate Volume
V_b = UreaGel Buffer Volume
V_d = UreaGel Diluent Volume
V_t = Total Casting Solution Volume
X = % Gel Desired

EXAMPLE:

To make 100ml of an 8% gel, calculate the UreaGel solution volume to be added as follows:
V_c =(100) (8)/25 = 32ml UreaGel 29:1 Concentrate
V_b = 0.1 (100) = 10ml UreaGel Buffer
V_d = 100 – (32 + 10) = 58ml UreaGel Diluent

Add Initiators and Cast Gel

Add 40 microliters of TEMED for every 100 ml of gel casting solution. Swirl gently to mix. Add 0.8 ml of FRESHLY PREPARED 10% ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Cast the gel. Insert the comb and allow to polymerize one to two hours.

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